RNA sequencing (polyA) report



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1. Introduction


RNA-Seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA in a biological sample at a given moment in time.

RNA-Seq is used to analyze the continuously changing cellular transcriptome. Specifically, RNA-Seq facilitates the ability to look at alternative gene spliced transcripts, post-transcriptional modifications, gene fusion, mutations/SNPs and changes in gene expression over time, or differences in gene expression in different groups or treatments. In addition to mRNA transcripts, RNA-Seq can look at different populations of RNA to include total RNA, small RNA, such as miRNA, tRNA, and ribosomal profiling. RNA-Seq can also be used to determine exon/intron boundaries and verify or amend previously annotated 5' and 3' gene boundaries

Prior to RNA-Seq, gene expression studies were done with hybridization-based microarrays. Issues with microarrays include cross-hybridization artifacts, poor quantification of lowly and highly expressed genes, and needing to know the sequence a priori. Because of these technical issues, transcriptomics transitioned to sequencing-based methods. These progressed from Sanger sequencing of Expressed Sequence Tag libraries, to chemical tag-based methods (e.g., serial analysis of gene expression), and finally to the current technology, next-gen sequencing of cDNA (notably RNA-Seq).


Fig.1 Summary of RNA-Seq


As shown in Fig.1, within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism, fragmented and copied into stable ds-cDNA (blue). The ds-cDNA is sequenced using high-throughput, short-read sequencing methods. These sequences can then be aligned to a reference genome sequence to reconstruct which genome regions were being transcribed. This data can be used to annotate where expressed genes are, their relative expression levels, and any alternative splice variants.



2. Materials and Methods


2.1 Library construction and sequencing


Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quality and quantity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Approximately 10 ug of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). Following purification, the poly(A) mRNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with a strand-specific library preparation by dUTP method. The average insert size for the paired-end libraries was 300±50 bp. And then we performed the paired-end 2×150bp sequencing on an Illumina Hiseq 4000 at LC Sciences following the vendor's recommended protocol.



2.2 Bioinformatics analysis


Transcripts Assembly:

Firstly, Cutadapt[1] and perl scripts in house were used to remove the reads that contained adaptor contamination, low quality bases and undetermined bases. Then sequence quality was verified using FastQC (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/). We used HISAT2[2] to map reads to the genome of Oryza sativa (Version: RGAP 7). The mapped reads of each sample were assembled using StringTie[3]. Then, all transcriptomes from 6 Samples were merged to reconstruct a comprehensive transcriptome using perl scripts and gffcompare (https://github.com/gpertea/gffcompare/). After the final transcriptome was generated, StringTie[3] and Ballgown[4] was used to estimate the expression levels of all transcripts.


Different expression analysis of mRNAs:

StringTie[3] was used to perform expression level for mRNAs by calculating FPKM (FPKM=[total_exon_fragments/mapped_reads(millions)×exon_length(kB)]). The differentially expressed mRNAs were selected with log2 (fold change) >1 or log2 (fold change) <-1 and with parametric F-test comparing nested linear models (p value < 0.05) by R package Ballgown[4].




Bioinformatics pipeline for RNA sequencing (polyA)


Reference:

[1] Martin M. Cutadapt removes adapter sequences from high-throughput sequencing reads. Embnet Journal 17 (2011).

[2] Kim, D., Langmead, B. & Salzberg, S.L. HISAT: a fast spliced aligner with low memory requirements. Nature Methods 12, 357–360 (2015).

[3] Pertea, M. et al. StringTie enables improved reconstruction of a transcriptome from RNA-seq reads. Nature Biotechnology. 33, 290–295 (2015).

[4] Frazee, A.C. et al. Ballgown bridges the gap between transcriptome assembly and expression analysis. Nature Biotechnology. 33, 243–246 (2015).




3. Project information


3.1 Sample information


Species name: Rice

Latin name: Oryza sativa

Specimens: ovule


3.2 Group information


SampleCOND1
Lo7_1Lo7
Lo7_2Lo7
Lo7_3Lo7
Lo8_1Lo8
Lo8_2Lo8
Lo8_3Lo8

3.3 Database


Dababase Web links Version/date
Genomehttp://rice.plantbiology.msu.edu/RGAP 7
Gene Orthology (GO)https://www.ncbi.nlm.nih.gov/projects/SNP/2016.12
KEGGhttp://www.kegg.jp/2017.06

3.4 Bioinformatics software


Analysis item Software Version/date
Quality controlFastQC0.10.1
Adapter removeCutadapt1.10
MappingHISAT2.0
Transcripts assemblyStringTie1.3
Differential expression analysisBallgown2016.09.29
GO and KEGG enrichment analysisPerl scripts in houseNA
SNP/Indel analysissamtools0.7
SNP/Indel annotationANNOVAR2017.09
Alternative splicingASprofile1.0



4. Results of standard analysis


4.1 Quality control and statistics of Reads


Statistics of reads from 6 libraries was shown below. Results of FastQC, PCA analysis and sample correlation were deposited in summary/8_Quality_Control.


SampleRaw DataValid DataValid Ratio(reads)Q20%Q30%GC content%
ReadBaseReadBase
Lo7_1439882866.60G436682426.55G99.2798.9991.4551
Lo7_2473477127.10G469960727.05G99.2699.1292.3451.50
Lo7_3447634286.71G444226806.66G99.2499.2392.6451.50
Lo8_1439910886.60G436718666.55G99.2799.2192.9751.50
Lo8_2456429846.85G453400746.80G99.3499.3492.7452
Lo8_3466785527.00G463544686.95G99.3199.0191.0950.50

document location: summary/1_rawdata/ReadsQC.xlsx


4.2 Mapping to genome


SampleValid readsMapped readsUnique Mapped readsMulti Mapped readsPE Mapped readsReads map to sense strandReads map to antisense strandNon-splice readsSplice reads
Lo7_14366824240525689(92.80%)32875835(75.29%)7649854(17.52%)37309218(85.44%)19379564(44.38%)19674393(45.05%)26202698(60.00%)12851259(29.43%)
Lo7_24699607243804863(93.21%)34574453(73.57%)9230410(19.64%)40581704(86.35%)20715701(44.08%)21069668(44.83%)28516068(60.68%)13269301(28.23%)
Lo7_34442268041579830(93.60%)32839571(73.93%)8740259(19.68%)38839830(87.43%)19721984(44.40%)20032131(45.09%)27193119(61.21%)12560996(28.28%)
Lo8_14367186640934692(93.73%)32505589(74.43%)8429103(19.30%)37878490(86.73%)19392874(44.41%)19689746(45.09%)26159989(59.90%)12922631(29.59%)
Lo8_24534007442486373(93.71%)33664651(74.25%)8821722(19.46%)39600686(87.34%)20152040(44.45%)20470043(45.15%)26981136(59.51%)13640947(30.09%)
Lo8_34635446842647519(92.00%)35043515(75.60%)7604004(16.40%)39000572(84.14%)20342722(43.89%)20631931(44.51%)26992873(58.23%)13981780(30.16%)

document location: summary/1_rawdata/2_mapped_stat.xlsx


4.3 Statistics of mapped region



document location: summary/2_reference_mapping/3_mapped_region_stat.png


4.4 Localization of mRNAs on genome





document location: summary/2_reference_mapping/4_DensityDistribution/*_chromosome.jpeg


4.5 Prediction of novel transcripts


Those transcripts assembled by StringTie which are not annotated in genome annotation database are belong to novel transcripts with class code (i, j, o, u ,x).


Class code typies and definitions:

class codeDescription
jPotentially novel isoform (fragment): at least one splice junction is shared with a reference transcript
iA transfrag falling entirely within a reference intron
oGeneric exonic overlap with a reference transcript
uUnknown, intergenic transcript
xExonic overlap with reference on the opposite strand

document location:

summary/3_novel_transcripts/novel_merged.fa

summary/3_novel_transcripts/novel_merged.gtf


Tips: The Gene transfer format (GTF) is a file format used to hold information about gene structure. It is a tab-delimited text format based on the general feature format (GFF), but contains some additional conventions specific to gene information. A significant feature of the GTF that can be validated: given a sequence and a GTF file, one can check that the format is correct. This significantly reduces problems with the interchange of data between groups. We recommend you to open files with gtf and fa format by using several text editors such as Notepad++ (https://notepad-plus-plus.org/) and Vim (http://www.vim.org/).


4.6 Overview of gene expression


StringTie was utilized to assemble all trascripts from mapped reads (bam format) by mapping software Tophat or HISAT2. Also both gene level and transcript level were estimated by StringTie using FPKM (FPKM=[total_exon_fragments/mapped_reads(millions)×exon_length(kB)]).


4.6.1 Statistics of gene/transcript expression


Statistics of gene expression:

SampleExp geneMin.1st Qu.MedianMean3rd Qu.Max.Sd.Sum.
Lo7_1285920.000.653.0912.7210.663829.0453.06363662.40
Lo7_2288700.000.602.8712.189.922509.1648.10351604.59
Lo7_3286380.000.602.8612.529.883423.9252.08358608.39
Lo8_1285210.000.673.0912.6610.192672.8249.89361048.01
Lo8_2287180.000.673.0012.9010.043714.3954.64370343.89
Lo8_3288100.000.653.2212.6510.662940.3049.43364377.26

Statistics of transcript expression:

SampleExp transcriptMin.1st Qu.MedianMean3rd Qu.Max.Sd.Sum.
Lo7_1576810.000.401.427.444.623829.0440.37429233.78
Lo7_2578480.000.371.347.254.315038.2641.32419308.11
Lo7_3574610.000.361.327.444.384606.1443.58427365.34
Lo8_1569120.000.371.337.544.413468.6441.59429105.86
Lo8_2573110.000.371.327.694.365177.1047.30440912.61
Lo8_3581770.000.391.417.394.612940.3038.04429953.33



document location:

summary/4_transcript_expression/1_gene_expression_statistics.xlsx

summary/4_transcript_expression/*_gene_expression_boxplot.png

summary/4_transcript_expression/1_gene_expression_statistics.xlsx

summary/4_transcript_expression/*_transcript_expression_boxplot.png


4.6.2 FPKM Interval (FI) of gene/transcripts expression


FI of gene expression:

Sample0-0.1 FI0.1-0.3 FI0.3-3.57 FI3.57-15 FI15-60 FI>60 FI
Lo7_12431(8.50%)2419(8.46%)10182(35.61%)8318(29.09%)4173(14.59%)1069(3.74%)
Lo7_22595(8.99%)2541(8.80%)10469(36.26%)8247(28.57%)4016(13.91%)1002(3.47%)
Lo7_32628(9.18%)2411(8.42%)10482(36.60%)8127(28.38%)3930(13.72%)1060(3.70%)
Lo8_12377(8.33%)2309(8.10%)10355(36.31%)8456(29.65%)3997(14.01%)1027(3.60%)
Lo8_22305(8.03%)2373(8.26%)10639(37.05%)8441(29.39%)3858(13.43%)1102(3.84%)
Lo8_32597(9.01%)2351(8.16%)9958(34.56%)8627(29.94%)4226(14.67%)1051(3.65%)

FI of transcripts expression:

Sample0-0.1 FI0.1-0.3 FI0.3-3.57 FI3.57-15 FI15-60 FI>60 FI
Lo7_16003(10.41%)6183(10.72%)28415(49.26%)11528(19.99%)4423(7.67%)1129(1.96%)
Lo7_26312(10.91%)6545(11.31%)28570(49.39%)11113(19.21%)4225(7.30%)1083(1.87%)
Lo7_36455(11.23%)6346(11.04%)28269(49.20%)11068(19.26%)4220(7.34%)1103(1.92%)
Lo8_16191(10.88%)6362(11.18%)28023(49.24%)10994(19.32%)4207(7.39%)1135(1.99%)
Lo8_26154(10.74%)6429(11.22%)28430(49.61%)10925(19.06%)4195(7.32%)1178(2.06%)
Lo8_36302(10.83%)6215(10.68%)28457(48.91%)11654(20.03%)4435(7.62%)1114(1.91%)

document location:

summary/4_transcript_expression/2_gene_expression_interval.xlsx

summary/4_transcript_expression/2_transcript_expression_interval.xlsx


4.6.3 Density of gene/transcript expression




document location:

summary/4_transcript_expression/*_gene_expression_density.png

summary/4_transcript_expression/*_transcript_expression_density.png


4.6.4 Gene/transcript expression profiling


Gene expression profiling:

gene_idgene_nametranscript_idGOKEGGKO_ENTRYECDescriptionFPKM.Lo7_1FPKM.Lo7_2FPKM.Lo7_3FPKM.Lo8_1FPKM.Lo8_2FPKM.Lo8_3
LOC_Os01g01090LOC_Os01g01090LOC_Os01g01090.1GO:0003755(peptidyl-prolyl cis-trans isomerase activity);GO:0005528(FK506 binding);GO:0005789(endoplasmic reticulum membrane);GO:0061077(chaperone-mediated protein folding)NANANAretrotransposon protein, putative, unclassified, expressed00000.020
LOC_Os01g01110LOC_Os01g01110LOC_Os01g01110.1GO:0004672(protein kinase activity);GO:0004674(protein serine/threonine kinase activity);GO:0005524(ATP binding);GO:0005886(plasma membrane);GO:0006468(protein phosphorylation);GO:0007169(transmembrane receptor protein tyrosine kinase signaling pathway);GO:0016020(membrane);GO:0016021(integral component of membrane);GO:0016301(kinase activity)04075(Plant hormone signal transduction);04626(Plant-pathogen interaction)K13416EC:2.7.10.1 2.7.11.1expressed protein0000.5900
LOC_Os01g01290LOC_Os01g01290LOC_Os01g01290.1GO:0003677(DNA binding);GO:0003700(transcription factor activity, sequence-specific DNA binding);GO:0005515(protein binding);GO:0005634(nucleus);GO:0005829(cytosol);GO:0006351(transcription, DNA-templated);GO:0006355(regulation of transcription, DNA-templated);GO:0009738(abscisic acid-activated signaling pathway);GO:0010468(regulation of gene expression);GO:0046982(protein heterodimerization activity);GO:0048586(regulation of long-day photoperiodism, flowering);GO:0051247(positive regulation of protein metabolic process);GO:2000905(negative regulation of starch metabolic process)NANANAhistone-like transcription factor and archaeal histone, putative, expressed00.110000
LOC_Os01g01360LOC_Os01g01360LOC_Os01g01360.1GO:0005215(transporter activity);GO:0005886(plasma membrane);GO:0006857(oligopeptide transport);GO:0016020(membrane);GO:0016021(integral component of membrane)NANANApeptide transporter PTR2, putative, expressed00.330.250.980.470.78
LOC_Os01g01470LOC_Os01g01470LOC_Os01g01470.1GO:0003677(DNA binding);GO:0003700(transcription factor activity, sequence-specific DNA binding);GO:0005634(nucleus);GO:0005737(cytoplasm);GO:0006351(transcription, DNA-templated);GO:0006355(regulation of transcription, DNA-templated);GO:0007275(multicellular organism development)03018(RNA degradation)K12581NAno apical meristem protein, putative, expressed0000.080.060
LOC_Os01g01730LOC_Os01g01730LOC_Os01g01730.1NANANANAserine/arginine repetitive matrix protein 2, putative, expressed000000.04
LOC_Os01g01770LOC_Os01g01770LOC_Os01g01770.1GO:0003676(nucleic acid binding);GO:0004527(exonuclease activity);GO:0005622(intracellular);GO:0005634(nucleus);GO:0046872(metal ion binding)03008(Ribosome biogenesis in eukaryotes)K14570EC:3.1.-.-ZOS1-01 - C2H2 zinc finger protein, expressed0.260.040.070.270.050
LOC_Os01g01840LOC_Os01g01840LOC_Os01g01840.1GO:0000977(RNA polymerase II regulatory region sequence-specific DNA binding);GO:0003700(transcription factor activity, sequence-specific DNA binding);GO:0006357(regulation of transcription from RNA polymerase II promoter);GO:0090575(RNA polymerase II transcription factor complex)NANANAhelix-loop-helix DNA-binding domain containing protein, expressed0.020.02000.050
LOC_Os01g01950LOC_Os01g01950LOC_Os01g01950.1NANANANAexpressed protein00.070.0700.210
LOC_Os01g01980LOC_Os01g01980LOC_Os01g01980.1NANANANAexpressed protein0000.1600
LOC_Os01g01990LOC_Os01g01990LOC_Os01g01990.1NANANANAexpressed protein0.2900000
LOC_Os01g02110LOC_Os01g02110LOC_Os01g02110.1GO:0001046(core promoter sequence-specific DNA binding);GO:0001228(transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding);GO:0005634(nucleus);GO:0006366(transcription from RNA polymerase II promoter)04075(Plant hormone signal transduction);04712(Circadian rhythm - plant)K12126NAhelix-loop-helix DNA-binding domain containing protein, expressed00.030000.08
LOC_Os01g02120LOC_Os01g02120LOC_Os01g02120.1GO:0005634(nucleus);GO:0005737(cytoplasm);GO:0008429(phosphatidylethanolamine binding);GO:0009737(response to abscisic acid);GO:0009738(abscisic acid-activated signaling pathway);GO:0010030(positive regulation of seed germination)04712(Circadian rhythm - plant)K16223NAosMFT2 MFT-Like2 homologous to Mother of FT and TFL1 gene; contains Pfam profile PF01161: Phosphatidylethanolamine-binding protein, expressed000000.05
LOC_Os01g02190LOC_Os01g02190LOC_Os01g02190.1GO:0005887(integral component of plasma membrane);GO:0009992(cellular water homeostasis);GO:0015250(water channel activity);GO:0015254(glycerol channel activity);GO:0034220(ion transmembrane transport)NANANAaquaporin protein, putative, expressed0.1700.170.120.080.19
LOC_Os01g02210LOC_Os01g02210LOC_Os01g02210.1GO:0003755(peptidyl-prolyl cis-trans isomerase activity);GO:0005528(FK506 binding);GO:0005789(endoplasmic reticulum membrane);GO:0061077(chaperone-mediated protein folding)NANANAretrotransposon protein, putative, Ty3-gypsy subclass000.00000
LOC_Os01g02250LOC_Os01g02250LOC_Os01g02250.1GO:0005515(protein binding);GO:0005524(ATP binding);GO:0005576(extracellular region);GO:0006952(defense response);GO:0043531(ADP binding)04626(Plant-pathogen interaction)K13457NARGA-1, putative, expressed000.01000.06
LOC_Os01g02310LOC_Os01g02310LOC_Os01g02310.1GO:0004674(protein serine/threonine kinase activity);GO:0005886(plasma membrane);GO:0006468(protein phosphorylation)04626(Plant-pathogen interaction)K13430EC:2.7.11.1expressed protein00.030000
LOC_Os01g02370LOC_Os01g02370LOC_Os01g02370.1GO:0005886(plasma membrane)00520(Amino sugar and nucleotide sugar metabolism)K01183EC:3.2.1.14receptor-like kinase ARK1AS, putative, expressed0.020.010.010.0900.04
LOC_Os01g02710LOC_Os01g02710LOC_Os01g02710.1GO:0005886(plasma membrane)NANANALRk-type protein, putative, expressed0.070.0300.0300.08
LOC_Os01g02950LOC_Os01g02950LOC_Os01g02950.1NANANANAexpressed protein00000.080
LOC_Os01g03260LOC_Os01g03260LOC_Os01g03260.1NANANANAexpressed protein000.03000
LOC_Os01g03270LOC_Os01g03270LOC_Os01g03270.1GO:0005634(nucleus);GO:0005737(cytoplasm);GO:0006915(apoptotic process);GO:0042787(protein ubiquitination involved in ubiquitin-dependent protein catabolic process);GO:0043161(proteasome-mediated ubiquitin-dependent protein catabolic process);GO:0061630(ubiquitin protein ligase activity)04120(Ubiquitin mediated proteolysis)K04506EC:2.3.2.27expressed protein0.120.100.020.490.270.20
LOC_Os01g03290LOC_Os01g03290LOC_Os01g03290.1GO:0005634(nucleus);GO:0005737(cytoplasm);GO:0006915(apoptotic process);GO:0042787(protein ubiquitination involved in ubiquitin-dependent protein catabolic process);GO:0043161(proteasome-mediated ubiquitin-dependent protein catabolic process);GO:0061630(ubiquitin protein ligase activity)04120(Ubiquitin mediated proteolysis)K04506EC:2.3.2.27seven in absentia protein family domain containing protein, expressed00000.170
LOC_Os01g03300LOC_Os01g03300LOC_Os01g03300.1GO:0005634(nucleus);GO:0005737(cytoplasm);GO:0006915(apoptotic process);GO:0042787(protein ubiquitination involved in ubiquitin-dependent protein catabolic process);GO:0043161(proteasome-mediated ubiquitin-dependent protein catabolic process);GO:0061630(ubiquitin protein ligase activity)04120(Ubiquitin mediated proteolysis)K04506EC:2.3.2.27seven in absentia protein family protein, expressed0.020.060.17000
LOC_Os01g03320LOC_Os01g03320LOC_Os01g03320.1NANANANABBTI2 - Bowman-Birk type bran trypsin inhibitor precursor, expressed0.740.810.780.870.200.70
LOC_Os01g03380LOC_Os01g03380LOC_Os01g03380.1NANANANABBTI6 - Bowman-Birk type bran trypsin inhibitor precursor, putative, expressed000.3200.060.12
LOC_Os01g03429LOC_Os01g03429LOC_Os01g03429.1GO:0005634(nucleus);GO:0005737(cytoplasm);GO:0006915(apoptotic process);GO:0042787(protein ubiquitination involved in ubiquitin-dependent protein catabolic process);GO:0043161(proteasome-mediated ubiquitin-dependent protein catabolic process);GO:0061630(ubiquitin protein ligase activity)04120(Ubiquitin mediated proteolysis)K04506EC:2.3.2.27expressed protein0.100.0400.0600.12
LOC_Os01g03464LOC_Os01g03464LOC_Os01g03464.1GO:0005634(nucleus);GO:0005737(cytoplasm);GO:0006915(apoptotic process);GO:0042787(protein ubiquitination involved in ubiquitin-dependent protein catabolic process);GO:0043161(proteasome-mediated ubiquitin-dependent protein catabolic process);GO:0061630(ubiquitin protein ligase activity)04120(Ubiquitin mediated proteolysis)K04506EC:2.3.2.27expressed protein00.040000.03
LOC_Os01g03620LOC_Os01g03620LOC_Os01g03620.1GO:0005507(copper ion binding);GO:0016722(oxidoreductase activity, oxidizing metal ions)NANANAmulticopper oxidase domain containing protein, expressed0.380.280.490.110.140.47
LOC_Os01g03670LOC_Os01g03670LOC_Os01g03670.1GO:0003824(catalytic activity);GO:0005829(cytosol);GO:0010584(pollen exine formation);GO:0016491(oxidoreductase activity);GO:0050662(coenzyme binding);GO:0055114(oxidation-reduction process);GO:0080110(sporopollenin biosynthetic process)00940(Phenylpropanoid biosynthesis)K09753EC:1.2.1.44dihydroflavonol-4-reductase, putative, expressed0.050.150.080.070.040.04
LOC_Os01g03840LOC_Os01g03840LOC_Os01g03840.1GO:0003700(transcription factor activity, sequence-specific DNA binding);GO:0005634(nucleus);GO:0008285(negative regulation of cell proliferation);GO:0010094(specification of carpel identity);GO:0010097(specification of stamen identity);GO:0043565(sequence-specific DNA binding);GO:0044212(transcription regulatory region DNA binding);GO:0048437(floral organ development)03410(Base excision repair)K10773EC:4.2.99.18ZOS1-02 - C2H2 zinc finger protein, expressed0.130.140.480.710.640.19
LOC_Os01g03890LOC_Os01g03890LOC_Os01g03890.1GO:0009739(response to gibberellin)NANANADUF260 domain containing protein, putative, expressed0.000.22000.020.07
LOC_Os01g03900LOC_Os01g03900LOC_Os01g03900.1NANANANAexpressed protein001.58000
LOC_Os01g03930LOC_Os01g03930LOC_Os01g03930.1NANANANAexpressed protein00.470000
LOC_Os01g03960LOC_Os01g03960LOC_Os01g03960.1NANANANAexpressed protein0000.1200
LOC_Os01g04005LOC_Os01g04005LOC_Os01g04005.1NANANANAexpressed protein0.010.010000
LOC_Os01g04090LOC_Os01g04090LOC_Os01g04090.1NANANANAexpressed protein0.370.630.600.430.430.51
LOC_Os01g04210LOC_Os01g04210LOC_Os01g04210.1GO:0008375(acetylglucosaminyltransferase activity);GO:0016021(integral component of membrane);GO:0016757(transferase activity, transferring glycosyl groups)04144(Endocytosis)K12471NADNA binding protein, putative, expressed0.380.0600.210.120.25
LOC_Os01g04220LOC_Os01g04220LOC_Os01g04220.1GO:0008375(acetylglucosaminyltransferase activity);GO:0016021(integral component of membrane);GO:0016757(transferase activity, transferring glycosyl groups)04144(Endocytosis)K12471NADNA binding protein, putative, expressed0.060.160.120.040.200.19
LOC_Os01g04250LOC_Os01g04250LOC_Os01g04250.1GO:0007165(signal transduction);GO:0016020(membrane)NANANAexpressed protein00.210.260.5600
LOC_Os01g04540LOC_Os01g04540LOC_Os01g04540.1NANANANAserine/threonine-protein kinase At1g18390 precursor, putative, expressed0.280.130.550.120.400.04
LOC_Os01g04550LOC_Os01g04550LOC_Os01g04550.1GO:0004674(protein serine/threonine kinase activity);GO:0005524(ATP binding);GO:0005886(plasma membrane);GO:0006468(protein phosphorylation);GO:0016021(integral component of membrane);GO:0016301(kinase activity);GO:0016310(phosphorylation)00520(Amino sugar and nucleotide sugar metabolism)K01183EC:3.2.1.14expressed protein0.220.330.310.170.210.35
LOC_Os01g04560LOC_Os01g04560LOC_Os01g04560.1GO:0004674(protein serine/threonine kinase activity);GO:0005886(plasma membrane);GO:0007166(cell surface receptor signaling pathway)NANANAexpressed protein0.050.200.02000
LOC_Os01g04640LOC_Os01g04640LOC_Os01g04640.1GO:0003674(molecular_function);GO:0005739(mitochondrion);GO:0005783(endoplasmic reticulum);GO:0005789(endoplasmic reticulum membrane);GO:0008150(biological_process);GO:0016021(integral component of membrane)NANANAreticulon domain containing protein, putative, expressed0.510.520.230.850.490.48
LOC_Os01g04699LOC_Os01g04699LOC_Os01g04699.1NANANANAexpressed protein0.150.2400.210.200.13
LOC_Os01g04750LOC_Os01g04750LOC_Os01g04750.1GO:0003677(DNA binding);GO:0003700(transcription factor activity, sequence-specific DNA binding);GO:0005634(nucleus);GO:0006351(transcription, DNA-templated);GO:0009873(ethylene-activated signaling pathway);GO:0045892(negative regulation of transcription, DNA-templated)NANANAB3 DNA binding domain containing protein, expressed0000.0300.09
LOC_Os01g04800LOC_Os01g04800LOC_Os01g04800.1GO:0003677(DNA binding);GO:0003700(transcription factor activity, sequence-specific DNA binding);GO:0005634(nucleus);GO:0006351(transcription, DNA-templated);GO:0006355(regulation of transcription, DNA-templated);GO:0009873(ethylene-activated signaling pathway);GO:0048573(photoperiodism, flowering)04075(Plant hormone signal transduction)K14486NAB3 DNA binding domain containing protein, expressed0.770.470.260.250.230.63
LOC_Os01g05050LOC_Os01g05050LOC_Os01g05050.1GO:0003674(molecular_function);GO:0005739(mitochondrion);GO:0005759(mitochondrial matrix);GO:0008150(biological_process)NANANAmitochondrial glycoprotein, putative, expressed000000.06
LOC_Os01g05120LOC_Os01g05120LOC_Os01g05120.1GO:0003674(molecular_function);GO:0005829(cytosol);GO:0006511(ubiquitin-dependent protein catabolic process);GO:0009507(chloroplast)04141(Protein processing in endoplasmic reticulum)K14016NAubiquitin fusion degradation protein, putative, expressed0.21000.120.130.18
LOC_Os01g05340LOC_Os01g05340LOC_Os01g05340.1NANANANAexpressed protein0.170.080.180.190.190.08
LOC_Os01g05360LOC_Os01g05360LOC_Os01g05360.1GO:0009834(plant-type secondary cell wall biogenesis);GO:0010417(glucuronoxylan biosynthetic process);GO:0042285(xylosyltransferase activity)NANANAexpressed protein00000.250
LOC_Os01g05380LOC_Os01g05380LOC_Os01g05380.1NANANANAexpressed protein000.02000
LOC_Os01g05400LOC_Os01g05400LOC_Os01g05400.1GO:0009834(plant-type secondary cell wall biogenesis);GO:0010417(glucuronoxylan biosynthetic process);GO:0042285(xylosyltransferase activity)NANANAexpressed protein000.05000
LOC_Os01g05440LOC_Os01g05440LOC_Os01g05440.1GO:0004526(ribonuclease P activity);GO:0005655(nucleolar ribonuclease P complex);GO:0090502(RNA phosphodiester bond hydrolysis, endonucleolytic)NANANAXH domain containing protein, expressed0.090.340.020.170.140.11
LOC_Os01g05450LOC_Os01g05450LOC_Os01g05450.1NANANANAexpressed protein0.450.130.120.240.130
LOC_Os01g05510LOC_Os01g05510LOC_Os01g05510.4NANANANAexpressed protein000000.02
LOC_Os01g05530LOC_Os01g05530LOC_Os01g05530.1GO:0000027(ribosomal large subunit assembly);GO:0003723(RNA binding);GO:0005730(nucleolus)NANANAexpressed protein000000.06
LOC_Os01g05580LOC_Os01g05580LOC_Os01g05580.1NANANANAExo70 exocyst complex subunit family, putative, expressed0000.0500
LOC_Os01g05585LOC_Os01g05585LOC_Os01g05585.1NANANANAmetallothionein, putative, expressed00.1000.3700.25
LOC_Os01g05600LOC_Os01g05600LOC_Os01g05600.1GO:0005515(protein binding);GO:0005524(ATP binding);GO:0005576(extracellular region);GO:0006952(defense response);GO:0043531(ADP binding)04626(Plant-pathogen interaction)K13457NANBS-LRR disease resistance protein, putative, expressed0000.4200
LOC_Os01g06030LOC_Os01g06030LOC_Os01g06030.1GO:0005634(nucleus)00350(Tyrosine metabolism);00950(Isoquinoline alkaloid biosynthesis)K01592EC:4.1.1.25expressed protein0000.0700
LOC_Os01g06040LOC_Os01g06040LOC_Os01g06040.1NA00350(Tyrosine metabolism);00950(Isoquinoline alkaloid biosynthesis)K01592EC:4.1.1.25expressed protein00.090000.10
LOC_Os01g06060LOC_Os01g06060LOC_Os01g06060.1GO:0005634(nucleus);GO:0008152(metabolic process);GO:0052689(carboxylic ester hydrolase activity)04075(Plant hormone signal transduction)K14493EC:3.-.-.-gibberellin receptor GID1L2, putative, expressed0.040.040.04000
LOC_Os01g06070LOC_Os01g06070LOC_Os01g06070.1NANANANAexpressed protein0.110.060000
LOC_Os01g06120LOC_Os01g06120LOC_Os01g06120.1NANANANAexpressed protein0.0300000
LOC_Os01g06150LOC_Os01g06150LOC_Os01g06150.1GO:0005634(nucleus)NANANAexpressed protein0.700.290.920.630.560.82
LOC_Os01g06210LOC_Os01g06210LOC_Os01g06210.1GO:0005634(nucleus);GO:0008152(metabolic process);GO:0052689(carboxylic ester hydrolase activity)04075(Plant hormone signal transduction)K14493EC:3.-.-.-gibberellin receptor GID1L2, putative, expressed0000.0000
LOC_Os01g06220LOC_Os01g06220LOC_Os01g06220.1GO:0005634(nucleus);GO:0008152(metabolic process);GO:0052689(carboxylic ester hydrolase activity)04075(Plant hormone signal transduction)K14493EC:3.-.-.-gibberellin receptor GID1L2, putative, expressed0.1800.040.070.120.10
LOC_Os01g06240LOC_Os01g06240LOC_Os01g06240.1GO:0004674(protein serine/threonine kinase activity);GO:0005886(plasma membrane);GO:0006468(protein phosphorylation)00260(Glycine, serine and threonine metabolism);00350(Tyrosine metabolism);00360(Phenylalanine metabolism);00410(beta-Alanine metabolism);00950(Isoquinoline alkaloid biosynthesis);00960(Tropane, piperidine and pyridine alkaloid biosynthesis)K00276EC:1.4.3.21protein kinase, putative, expressed0.080.06000.060
LOC_Os01g06370LOC_Os01g06370LOC_Os01g06370.1NANANANAexpressed protein00.34000.080
LOC_Os01g06390LOC_Os01g06390LOC_Os01g06390.1NANANANAexpressed protein000000.08
LOC_Os01g06400LOC_Os01g06400LOC_Os01g06400.1NANANANAexpressed protein0.030.110000
LOC_Os01g06420LOC_Os01g06420LOC_Os01g06420.1NANANANAexpressed protein00.1500.240.460.15
LOC_Os01g06430LOC_Os01g06430LOC_Os01g06430.1NANANANAexpressed protein0000.250.060
LOC_Os01g06540LOC_Os01g06540LOC_Os01g06540.1GO:0003677(DNA binding);GO:0005634(nucleus);GO:0005829(cytosol);GO:0006351(transcription, DNA-templated);GO:0006355(regulation of transcription, DNA-templated);GO:0008270(zinc ion binding);GO:0016036(cellular response to phosphate starvation);GO:0016568(chromatin modification);GO:0035064(methylated histone binding);GO:0042393(histone binding);GO:0048767(root hair elongation);GO:0055065(metal ion homeostasis)NANANAPHD finger protein, putative, expressed0.170.10000.260
LOC_Os01g06670LOC_Os01g06670LOC_Os01g06670.1GO:0006952(defense response);GO:0007165(signal transduction);GO:0016021(integral component of membrane);GO:0016301(kinase activity)04626(Plant-pathogen interaction)K13466NAhcr2-5D, putative, expressed0000.0200
LOC_Os01g06720LOC_Os01g06720LOC_Os01g06720.1GO:0006952(defense response);GO:0007165(signal transduction);GO:0016021(integral component of membrane);GO:0016301(kinase activity)04626(Plant-pathogen interaction)K13420EC:2.7.11.1disease resistance protein SlVe2 precursor, putative, expressed0.1400.090.040.020.01
LOC_Os01g06750LOC_Os01g06750LOC_Os01g06750.1GO:0005886(plasma membrane);GO:0006952(defense response);GO:0007165(signal transduction);GO:0016301(kinase activity)04075(Plant hormone signal transduction)K13415EC:2.7.10.1 2.7.11.1verticillium wilt disease resistance protein precursor, putative, expressed0.060.050.1100.160.14
LOC_Os01g06790LOC_Os01g06790LOC_Os01g06790.1GO:0006952(defense response);GO:0007165(signal transduction);GO:0016021(integral component of membrane);GO:0016301(kinase activity)04626(Plant-pathogen interaction)K13420EC:2.7.11.1disease resistance protein, putative, expressed0.020.080.030.0300.02
LOC_Os01g06820LOC_Os01g06820LOC_Os01g06820.1GO:0006952(defense response);GO:0007165(signal transduction);GO:0016021(integral component of membrane);GO:0016301(kinase activity)04626(Plant-pathogen interaction)K13420EC:2.7.11.1hcr2-0B, putative, expressed0.1500.100.100.130.05
LOC_Os01g06836LOC_Os01g06836LOC_Os01g06836.1GO:0005886(plasma membrane);GO:0006952(defense response);GO:0007165(signal transduction);GO:0016301(kinase activity)04626(Plant-pathogen interaction)K13466NAdisease resistance protein SlVe2 precursor, putative, expressed0.100.0500.050.030
LOC_Os01g06870LOC_Os01g06870LOC_Os01g06870.1GO:0006952(defense response);GO:0007165(signal transduction);GO:0016021(integral component of membrane)04626(Plant-pathogen interaction)K13420EC:2.7.11.1resistance protein SlVe1 precursor, putative, expressed00.030000.05
LOC_Os01g06920LOC_Os01g06920LOC_Os01g06920.1GO:0006952(defense response);GO:0007165(signal transduction);GO:0016021(integral component of membrane);GO:0016301(kinase activity)04626(Plant-pathogen interaction)K13466NAresistance protein SlVe1 precursor, putative, expressed0.160.3200.440.510.54
LOC_Os01g07020LOC_Os01g07020LOC_Os01g07020.1GO:0005887(integral component of plasma membrane);GO:0015179(L-amino acid transmembrane transporter activity);GO:0015297(antiporter activity)NANANAPOEI39 - Pollen Ole e I allergen and extensin family protein precursor, putative, expressed0.0900.04000
LOC_Os01g07030LOC_Os01g07030LOC_Os01g07030.1GO:0005887(integral component of plasma membrane);GO:0015179(L-amino acid transmembrane transporter activity);GO:0015297(antiporter activity)NANANAPOEI40 - Pollen Ole e I allergen and extensin family protein precursor, expressed0.030.120.210.090.180.32
LOC_Os01g07040LOC_Os01g07040LOC_Os01g07040.1GO:0005887(integral component of plasma membrane);GO:0015179(L-amino acid transmembrane transporter activity);GO:0015297(antiporter activity)NANANAPOEI41 - Pollen Ole e I allergen and extensin family protein precursor, expressed000000.12
LOC_Os01g07150LOC_Os01g07150LOC_Os01g07150.1GO:0003674(molecular_function);GO:0008150(biological_process)NANANAexpressed protein00000.080
LOC_Os01g07160LOC_Os01g07160LOC_Os01g07160.1GO:0004842(ubiquitin-protein transferase activity);GO:0019005(SCF ubiquitin ligase complex);GO:0031146(SCF-dependent proteasomal ubiquitin-dependent protein catabolic process)NANANAOsFBX3 - F-box domain containing protein, expressed0.210.28000.040
LOC_Os01g07180LOC_Os01g07180LOC_Os01g07180.1NANANANAexpressed protein000.100.1500.10
LOC_Os01g07364LOC_Os01g07364LOC_Os01g07364.1NANANANAexpressed protein000000.11
LOC_Os01g07430LOC_Os01g07430LOC_Os01g07430.1GO:0003677(DNA binding);GO:0003700(transcription factor activity, sequence-specific DNA binding);GO:0005634(nucleus);GO:0006355(regulation of transcription, DNA-templated);GO:0009651(response to salt stress);GO:0009723(response to ethylene);GO:0009737(response to abscisic acid);GO:0009753(response to jasmonic acid)00190(Oxidative phosphorylation);00860(Porphyrin and chlorophyll metabolism)K02259NAmyb-like DNA-binding domain containing protein, putative, expressed0.030.060.050.070.350.29
LOC_Os01g07450LOC_Os01g07450LOC_Os01g07450.1GO:0003677(DNA binding);GO:0005634(nucleus);GO:0006351(transcription, DNA-templated);GO:0006355(regulation of transcription, DNA-templated);GO:0045892(negative regulation of transcription, DNA-templated)00190(Oxidative phosphorylation);00860(Porphyrin and chlorophyll metabolism)K02259NAMYB family transcription factor, putative, expressed0.1300.130.060.060.03
LOC_Os01g07460LOC_Os01g07460LOC_Os01g07460.1NANANANAconserved hypothetical protein0000.1400
LOC_Os01g07550LOC_Os01g07550LOC_Os01g07550.1NANANANAhypothetical protein000000.06
LOC_Os01g07640LOC_Os01g07640LOC_Os01g07640.1GO:0005575(cellular_component);GO:0008150(biological_process)04075(Plant hormone signal transduction)K14500EC:2.7.11.1ankyrin, putative, expressed0.170.040.070.210.120.15
LOC_Os01g07660LOC_Os01g07660LOC_Os01g07660.1NANANANAexpressed protein00.050000
LOC_Os01g07710LOC_Os01g07710LOC_Os01g07710.1NANANANAexpressed protein000.060.040.230.14
LOC_Os01g07770LOC_Os01g07770LOC_Os01g07770.1GO:0004601(peroxidase activity);GO:0009505(plant-type cell wall);GO:0009664(plant-type cell wall organization)00940(Phenylpropanoid biosynthesis)K00430EC:1.11.1.7peroxidase precursor, putative, expressed0.380.690.560.270.450.63
LOC_Os01g08070LOC_Os01g08070LOC_Os01g08070.1GO:0000147(actin cortical patch assembly);GO:0005884(actin filament);GO:0006897(endocytosis);GO:0008360(regulation of cell shape);GO:0030048(actin filament-based movement);GO:0030479(actin cortical patch);GO:0051015(actin filament binding);GO:0051127(positive regulation of actin nucleation);GO:0051666(actin cortical patch localization)NANANAtransposon protein, putative, CACTA, En/Spm sub-class, expressed00.020000


Transcript expression profiling:

t_idclass_codechrstrandstartendt_namenum_exonslengthgene_idgene_nameGOKEGGKO_ENTRYECDescriptioncov.Lo7_1FPKM.Lo7_1cov.Lo7_2FPKM.Lo7_2cov.Lo7_3FPKM.Lo7_3cov.Lo8_1FPKM.Lo8_1cov.Lo8_2FPKM.Lo8_2cov.Lo8_3FPKM.Lo8_3
10chr1+290310817LOC_Os01g01010.1123017MSTRG.3LOC_Os01g01010GO:0005096(GTPase activator activity);GO:0005622(intracellular);GO:0006886(intracellular protein transport);GO:0012505(endomembrane system);GO:0017137(Rab GTPase binding);GO:0031338(regulation of vesicle fusion)03040(Spliceosome)K12840NATBC domain containing protein, expressed15.392.5933.215.2022.183.6522.483.7632.345.2030.974.95
2jchr1+292610822MSTRG.3.2122986MSTRG.3LOC_Os01g01010NANANANANA36.846.2026.394.1319.533.2120.503.4326.734.3038.236.12
3jchr1+293810822MSTRG.3.3122923MSTRG.3LOC_Os01g01010NANANANANA5.830.981.410.220.590.100.530.090000
4jchr1+296810811MSTRG.3.4132857MSTRG.3LOC_Os01g01010NANANANANA00000.870.14000000
50chr1+298410562LOC_Os01g01010.2122218MSTRG.3LOC_Os01g01010GO:0005096(GTPase activator activity);GO:0005622(intracellular);GO:0006886(intracellular protein transport);GO:0012505(endomembrane system);GO:0017137(Rab GTPase binding);GO:0031338(regulation of vesicle fusion)03040(Spliceosome)K12840NATBC domain containing protein, expressed0.090.020.050.010.000.000.010.00000.070.01
6jchr1+1108620323MSTRG.4.1136352MSTRG.4LOC_Os01g01040NANANANANA0031.284.9000000000
7jchr1+1108620323MSTRG.4.2136775MSTRG.4LOC_Os01g01040NANANANANA005.510.866.010.992.240.37004.060.65
80chr1+1121812435LOC_Os01g01019.121127MSTRG.4LOC_Os01g01019NANANANAexpressed protein0.750.13000.400.07000000
9jchr1+1262720377MSTRG.4.4115478MSTRG.4LOC_Os01g01040NANANANANA20.383.43009.411.557.621.270000
10jchr1+1262720377MSTRG.4.5125285MSTRG.4LOC_Os01g01040NANANANANA16.702.81000021.453.590017.342.77
110chr1+1264815915LOC_Os01g01030.152464MSTRG.4LOC_Os01g01030GO:0005507(copper ion binding);GO:0009505(plant-type cell wall);GO:0009506(plasmodesma);GO:0016722(oxidoreductase activity, oxidizing metal ions)00053(Ascorbate and aldarate metabolism)K00423EC:1.10.3.3monocopper oxidase, putative, expressed001.180.180000004.370.70
12jchr1+1265520323MSTRG.4.7105494MSTRG.4LOC_Os01g01040NANANANANA4.530.760.570.09002.190.3717.792.8632.295.16
13jchr1+1602420323MSTRG.4.892685MSTRG.4LOC_Os01g01040NANANANANA2.260.380.820.13004.490.758.141.3100
14jchr1+1602920320MSTRG.4.992674MSTRG.4LOC_Os01g01040NANANANANA3.050.511.670.26004.060.689.141.4700
150chr1+1629218304LOC_Os01g01040.431524MSTRG.4LOC_Os01g01040GO:0003674(molecular_function);GO:0005739(mitochondrion);GO:0008150(biological_process);GO:0016021(integral component of membrane)NANANAexpressed protein0.060.012.110.330.710.1200000.200.03
160chr1+1629220323LOC_Os01g01040.182508MSTRG.4LOC_Os01g01040GO:0003674(molecular_function);GO:0005739(mitochondrion);GO:0008150(biological_process);GO:0016021(integral component of membrane)NANANAexpressed protein1.650.288.441.3211.141.832.700.458.961.440.400.06
170chr1+1632120323LOC_Os01g01040.282482MSTRG.4LOC_Os01g01040GO:0003674(molecular_function);GO:0005739(mitochondrion);GO:0008150(biological_process);GO:0016021(integral component of membrane)NANANAexpressed protein1.150.194.180.6519.033.133.070.518.021.290.290.05
180chr1+1632120323LOC_Os01g01040.372583MSTRG.4LOC_Os01g01040GO:0003674(molecular_function);GO:0005739(mitochondrion);GO:0008150(biological_process);GO:0016021(integral component of membrane)NANANAexpressed protein1.160.202.920.4619.453.203.290.559.611.542.790.45
190chr1+2284126971LOC_Os01g01050.162039MSTRG.1LOC_Os01g01050NANANANAR3H domain containing protein, expressed16.812.833.310.526.551.086.471.088.231.320.850.14
200chr1+2284126971LOC_Os01g01050.261996MSTRG.1LOC_Os01g01050NANANANAR3H domain containing protein, expressed18.743.1611.281.7722.153.6521.033.5213.282.1427.274.36
21jchr1+2285026978MSTRG.1.352327MSTRG.1LOC_Os01g01050NANANANANA12.342.085.700.8913.122.162.090.3512.001.9326.444.23
220chr1+2713628651LOC_Os01g01060.14920MSTRG.2LOC_Os01g01060GO:0000028(ribosomal small subunit assembly);GO:0003729(mRNA binding);GO:0003735(structural constituent of ribosome);GO:0006412(translation);GO:0019843(rRNA binding);GO:0022627(cytosolic small ribosomal subunit)03010(Ribosome)K02989NA40S ribosomal protein S5, putative, expressed535.2790.14624.3697.77532.5887.67893.89149.45729.34117.29878.38140.51
23jchr1+2977334495MSTRG.5.1132115MSTRG.5LOC_Os01g01070NANANANANA8.021.358.851.3926.324.3323.843.9910.421.6810.811.73
24jchr1+2978934480MSTRG.5.2121994MSTRG.5LOC_Os01g01070NANANANANA9.221.5510.441.647.971.316.521.098.141.3113.222.12
250chr1+2981833943LOC_Os01g01070.3131369MSTRG.5LOC_Os01g01070GO:0003674(molecular_function);GO:0005739(mitochondrion);GO:0008150(biological_process);GO:0016021(integral component of membrane);GO:0031307(integral component of mitochondrial outer membrane)NANANAexpressed protein8.291.4010.251.606.701.108.341.398.651.395.190.83
260chr1+2981834493LOC_Os01g01070.1141568MSTRG.5LOC_Os01g01070GO:0003674(molecular_function);GO:0005739(mitochondrion);GO:0008150(biological_process);GO:0016021(integral component of membrane);GO:0031307(integral component of mitochondrial outer membrane)NANANAexpressed protein5.530.936.781.060.550.092.770.465.620.903.870.62
270chr1+2981834493LOC_Os01g01070.2141562MSTRG.5LOC_Os01g01070GO:0003674(molecular_function);GO:0005739(mitochondrion);GO:0008150(biological_process);GO:0016021(integral component of membrane);GO:0031307(integral component of mitochondrial outer membrane)NANANAexpressed protein78.9113.2969.3510.8642.817.0542.567.1246.447.4759.999.60
28jchr1+3539241235MSTRG.6.2132562MSTRG.6LOC_Os01g01080NANANANANA3.700.6220.493.214.220.69003.230.524.660.75
29jchr1+3539241235MSTRG.6.3132814MSTRG.6LOC_Os01g01080NANANANANA0.980.173.290.522.220.361.390.231.400.221.510.24
30jchr1+3539241253MSTRG.6.1142324MSTRG.6LOC_Os01g01080NANANANANA9.091.5318.912.9645.717.5212.152.0348.937.8712.562.01
31jchr1+3545041253MSTRG.6.4142266MSTRG.6LOC_Os01g01080NANANANANA12.742.1526.784.193.610.591.640.278.751.418.541.37
32jchr1+3557941188MSTRG.6.5142231MSTRG.6LOC_Os01g01080NANANANANA29.604.9817.972.8118.913.1129.444.9218.182.9242.956.87
33jchr1+3557941188MSTRG.6.6122466MSTRG.6LOC_Os01g01080NANANANANA1.650.280.650.102.130.358.521.421.370.229.941.59
340chr1+3558141180LOC_Os01g01080.1151955MSTRG.6LOC_Os01g01080GO:0005783(endoplasmic reticulum);GO:0005789(endoplasmic reticulum membrane);GO:0006520(cellular amino acid metabolic process);GO:0008117(sphinganine-1-phosphate aldolase activity);GO:0016020(membrane);GO:0016021(integral component of membrane);GO:0016831(carboxy-lyase activity);GO:0019752(carboxylic acid metabolic process);GO:0030149(sphingolipid catabolic process);GO:0030170(pyridoxal phosphate binding)00600(Sphingolipid metabolism)K01634EC:4.1.2.27decarboxylase, putative, expressed214.3136.09122.4419.17162.2126.70174.3729.15114.3618.3990.6114.49
350chr1+3558141180LOC_Os01g01080.2151976MSTRG.6LOC_Os01g01080GO:0005783(endoplasmic reticulum);GO:0005789(endoplasmic reticulum membrane);GO:0006520(cellular amino acid metabolic process);GO:0008117(sphinganine-1-phosphate aldolase activity);GO:0016020(membrane);GO:0016021(integral component of membrane);GO:0016831(carboxy-lyase activity);GO:0019752(carboxylic acid metabolic process);GO:0030149(sphingolipid catabolic process);GO:0030170(pyridoxal phosphate binding)00600(Sphingolipid metabolism)K01634EC:4.1.2.27decarboxylase, putative, expressed0.320.05000.260.042.120.350.270.048.331.33
360chr1+3558141180LOC_Os01g01080.3142042MSTRG.6LOC_Os01g01080GO:0005783(endoplasmic reticulum);GO:0005789(endoplasmic reticulum membrane);GO:0006520(cellular amino acid metabolic process);GO:0008117(sphinganine-1-phosphate aldolase activity);GO:0016020(membrane);GO:0016021(integral component of membrane);GO:0016831(carboxy-lyase activity);GO:0019752(carboxylic acid metabolic process);GO:0030149(sphingolipid catabolic process);GO:0030170(pyridoxal phosphate binding)00600(Sphingolipid metabolism)K01634EC:4.1.2.27decarboxylase, putative, expressed1.680.289.201.440.610.105.930.990.560.092.430.39
370chr1+4459547526LOC_Os01g01090.182409LOC_Os01g01090LOC_Os01g01090GO:0003755(peptidyl-prolyl cis-trans isomerase activity);GO:0005528(FK506 binding);GO:0005789(endoplasmic reticulum membrane);GO:0061077(chaperone-mediated protein folding)NANANAretrotransposon protein, putative, unclassified, expressed000000000.130.0200
380chr1+5708257972LOC_Os01g01110.11891LOC_Os01g01110LOC_Os01g01110GO:0004672(protein kinase activity);GO:0004674(protein serine/threonine kinase activity);GO:0005524(ATP binding);GO:0005886(plasma membrane);GO:0006468(protein phosphorylation);GO:0007169(transmembrane receptor protein tyrosine kinase signaling pathway);GO:0016020(membrane);GO:0016021(integral component of membrane);GO:0016301(kinase activity)04075(Plant hormone signal transduction);04626(Plant-pathogen interaction)K13416EC:2.7.10.1 2.7.11.1expressed protein0000003.550.590000
39jchr1+5843163576MSTRG.7.174147MSTRG.7LOC_Os01g01120NANANANANA5.800.987.881.234.570.7514.382.4018.272.9411.231.80
410chr1+6205963576LOC_Os01g01120.141206MSTRG.7LOC_Os01g01120GO:0000287(magnesium ion binding);GO:0005737(cytoplasm);GO:0005829(cytosol);GO:0008270(zinc ion binding);GO:0009507(chloroplast);GO:0009570(chloroplast stroma);GO:0016787(hydrolase activity);GO:0019284(L-methionine biosynthetic process from S-adenosylmethionine);GO:0019509(L-methionine biosynthetic process from methylthioadenosine);GO:0043715(2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity);GO:0043716(2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity);GO:0043874(acireductone synthase activity);GO:0046570(methylthioribulose 1-phosphate dehydratase activity)00270(Cysteine and methionine metabolism)K09880EC:3.1.3.77enolase-phosphatase E1, putative, expressed16.522.788.601.356.601.0916.682.7917.542.8225.994.16
420chr1-6324466302LOC_Os01g01130.172002MSTRG.8LOC_Os01g01130GO:0005634(nucleus)03013(RNA transport)K13151NAsnurportin-1, putative, expressed30.535.1427.094.2422.843.7631.685.3017.592.8340.196.43
440chr1+7277577699LOC_Os01g01150.133756MSTRG.10LOC_Os01g01150GO:0000166(nucleotide binding);GO:0003676(nucleic acid binding);GO:0003723(RNA binding);GO:0005634(nucleus);GO:0008150(biological_process);GO:0046872(metal ion binding)NANANARNA recognition motif, putative, expressed9.771.6413.052.0423.463.8618.373.070013.542.17
450chr1+7277578702LOC_Os01g01150.244638MSTRG.10LOC_Os01g01150GO:0000166(nucleotide binding);GO:0003676(nucleic acid binding);GO:0003723(RNA binding);GO:0005634(nucleus);GO:0008150(biological_process);GO:0046872(metal ion binding)NANANARNA recognition motif, putative, expressed4.770.804.980.784.530.752.790.471.210.196.331.01
460chr1+7277579938LOC_Os01g01150.354603MSTRG.10LOC_Os01g01150GO:0000166(nucleotide binding);GO:0003676(nucleic acid binding);GO:0003723(RNA binding);GO:0005634(nucleus);GO:0008150(biological_process);GO:0046872(metal ion binding)NANANARNA recognition motif, putative, expressed6.521.104.710.744.970.823.090.527.561.226.140.98
47jchr1+7277580049MSTRG.10.164241MSTRG.10LOC_Os01g01150NANANANANA16.722.8214.042.207.221.1912.962.1721.343.4314.082.25
48jchr1+7278679891MSTRG.10.663995MSTRG.10LOC_Os01g01150NANANANANA4.570.7711.711.8310.361.713.820.6410.381.677.531.21
49jchr1+7278679891MSTRG.10.773951MSTRG.10LOC_Os01g01150NANANANANA7.511.2711.361.7810.461.725.710.9516.772.7013.712.19
50jchr1+7278680049MSTRG.10.564239MSTRG.10LOC_Os01g01150NANANANANA11.051.8614.902.3310.331.705.230.877.971.288.751.40
51jchr1+7280479846MSTRG.10.854385MSTRG.10LOC_Os01g01150NANANANANA6.681.123.040.480023.873.991.430.237.191.15
52jchr1+7281780049MSTRG.10.1074001MSTRG.10LOC_Os01g01150NANANANANA7.491.268.431.328.691.432.190.374.900.794.690.75
53jchr1+7281780049MSTRG.10.973990MSTRG.10LOC_Os01g01150NANANANANA13.702.318.741.376.591.093.530.596.971.122.430.39
540chr1+8242884302LOC_Os01g01160.121090MSTRG.9LOC_Os01g01160GO:0005515(protein binding);GO:0005634(nucleus);GO:0006457(protein folding);GO:0009507(chloroplast);GO:0010322(regulation of isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway);GO:0061077(chaperone-mediated protein folding);GO:1902395(regulation of 1-deoxy-D-xylulose-5-phosphate synthase activity)04141(Protein processing in endoplasmic reticulum)K09518NAheat shock protein DnaJ, putative, expressed31.735.3429.184.5727.734.5634.855.8318.773.022.340.37
550chr1+8533788844LOC_Os01g01170.172334MSTRG.12LOC_Os01g01170NANANANAexpressed protein3.590.607.641.203.710.6111.931.9925.354.0815.252.44
56jchr1+8534088935MSTRG.12.272419MSTRG.12LOC_Os01g01170NANANANANA4.250.722.860.451.390.238.351.409.641.556.571.05
600chr1+134291135685LOC_Os01g01280.131226MSTRG.11LOC_Os01g01280GO:0003674(molecular_function);GO:0008150(biological_process);GO:0009507(chloroplast);GO:0009543(chloroplast thylakoid lumen);GO:0009579(thylakoid);GO:0031977(thylakoid lumen)NANANAexpressed protein9.521.6012.171.9112.452.057.221.215.420.8713.002.08
610chr1+139820141588LOC_Os01g01290.121556LOC_Os01g01290LOC_Os01g01290GO:0003677(DNA binding);GO:0003700(transcription factor activity, sequence-specific DNA binding);GO:0005515(protein binding);GO:0005634(nucleus);GO:0005829(cytosol);GO:0006351(transcription, DNA-templated);GO:0006355(regulation of transcription, DNA-templated);GO:0009738(abscisic acid-activated signaling pathway);GO:0010468(regulation of gene expression);GO:0046982(protein heterodimerization activity);GO:0048586(regulation of long-day photoperiodism, flowering);GO:0051247(positive regulation of protein metabolic process);GO:2000905(negative regulation of starch metabolic process)NANANAhistone-like transcription factor and archaeal histone, putative, expressed000.670.1100000000
620chr1+141936144554LOC_Os01g01295.141793MSTRG.15LOC_Os01g01295GO:0009507(chloroplast)NANANAexpressed protein47.467.9918.232.8657.909.5357.659.6448.857.8622.593.61
63jchr1+142782144556MSTRG.15.221644MSTRG.15LOC_Os01g01295NANANANANA8.711.477.811.227.221.197.181.205.970.962.500.40
640chr1+145577147852LOC_Os01g01302.191155MSTRG.16LOC_Os01g01302GO:0000287(magnesium ion binding);GO:0004765(shikimate kinase activity);GO:0009073(aromatic amino acid family biosynthetic process);GO:0019632(shikimate metabolic process)01230(Biosynthesis of amino acids);00400(Phenylalanine, tyrosine and tryptophan biosynthesis)K00891EC:2.7.1.71shikimate kinase, putative, expressed54.089.1144.186.9229.414.8443.917.3462.4910.0550.678.11
65jchr1+145602147854MSTRG.16.281228MSTRG.16LOC_Os01g01302NANANANANA1.320.226.230.9811.301.8613.632.280.270.0417.742.84
660chr1+148045150577LOC_Os01g01307.15979MSTRG.17LOC_Os01g01307GO:0003674(molecular_function);GO:0005576(extracellular region);GO:0005774(vacuolar membrane);GO:0005783(endoplasmic reticulum);GO:0005789(endoplasmic reticulum membrane);GO:0005794(Golgi apparatus);GO:0008150(biological_process);GO:0009506(plasmodesma);GO:0016021(integral component of membrane)04141(Protein processing in endoplasmic reticulum)K13250NAtranslocon-associated protein beta domain containing protein, expressed0000000030.114.8400
68jchr1+148055150610MSTRG.17.651004MSTRG.17LOC_Os01g01307NANANANANA38.596.50104.8916.4353.908.8728.364.7474.3511.9613.472.16
69jchr1+148055150610MSTRG.17.75988MSTRG.17LOC_Os01g01307NANANANANA1.550.267.511.186.511.0722.203.712.030.331.450.23
70jchr1+148055150610MSTRG.17.861353MSTRG.17LOC_Os01g01307NANANANANA12.362.0816.902.6536.656.0332.485.4314.592.3514.052.25
71jchr1+148055150643MSTRG.17.351037MSTRG.17LOC_Os01g01307NANANANANA127.4521.4632.255.0543.837.22154.0725.76101.6316.34157.6625.22
72jchr1+148055150643MSTRG.17.461037MSTRG.17LOC_Os01g01307NANANANANA153.8825.9147.097.3735.585.8695.2315.9299.6316.02164.7426.35
73jchr1+148055150643MSTRG.17.551386MSTRG.17LOC_Os01g01307NANANANANA22.533.7925.684.0225.344.1710.111.6911.201.8021.843.49
74jchr1+148068150571MSTRG.17.951429MSTRG.17LOC_Os01g01307NANANANANA36.586.1640.716.3857.139.4029.965.0148.797.8548.357.73
75jchr1+148072150567MSTRG.17.106800MSTRG.17LOC_Os01g01307NANANANANA0.930.162.630.410.820.1421.123.53000.490.08
760chr1+151409156500LOC_Os01g01312.174165MSTRG.14LOC_Os01g01312GO:0003677(DNA binding);GO:0004386(helicase activity);GO:0005524(ATP binding);GO:0005634(nucleus);GO:0006281(DNA repair);GO:0006351(transcription, DNA-templated);GO:0006355(regulation of transcription, DNA-templated);GO:0006974(cellular response to DNA damage stimulus);GO:0010332(response to gamma radiation)03420(Nucleotide excision repair)K10841NAretrotransposon protein, putative, Ty1-copia subclass, expressed30.905.2019.012.9830.104.9622.813.8136.345.8436.305.81
77xchr1+163052169672MSTRG.21.151539MSTRG.21LOC_Os01g01340NANANANANA14.812.4914.512.277.941.3119.493.2624.843.9917.762.84
78xchr1+163052169672MSTRG.21.241426MSTRG.21LOC_Os01g01340NANANANANA0011.001.72003.580.60006.120.98
79xchr1+163114169672MSTRG.21.351480MSTRG.21LOC_Os01g01340NANANANANA9.401.580013.352.201.840.3110.241.656.401.02
800chr1-168470170381LOC_Os01g01340.221731MSTRG.22LOC_Os01g01340GO:0003674(molecular_function);GO:0008150(biological_process);GO:0009507(chloroplast)04144(Endocytosis)K12198NAlight-induced protein 1-like, putative, expressed3.090.520018.783.0984.0614.051.050.170.570.09
810chr1-168470170389LOC_Os01g01340.131645MSTRG.22LOC_Os01g01340GO:0003674(molecular_function);GO:0008150(biological_process);GO:0009507(chloroplast)04144(Endocytosis)K12198NAlight-induced protein 1-like, putative, expressed360.5660.72263.5441.27145.2523.9142.257.0689.5814.41221.0335.36
82jchr1-168581173195MSTRG.22.383105MSTRG.22LOC_Os01g01350NANANANANA18.423.1023.383.668.461.3940.636.7938.286.1632.975.27
830chr1-170712173191LOC_Os01g01350.171151MSTRG.22LOC_Os01g01350GO:0005515(protein binding);GO:0005634(nucleus);GO:0005737(cytoplasm);GO:0007034(vacuolar transport);GO:0016192(vesicle-mediated transport)04144(Endocytosis)K12198NASNF7 domain containing protein, putative, expressed9.771.649.481.4815.882.6122.293.736.721.0832.125.14
840chr1-170712173434LOC_Os01g01350.251574MSTRG.22LOC_Os01g01350GO:0005515(protein binding);GO:0005634(nucleus);GO:0005737(cytoplasm);GO:0007034(vacuolar transport);GO:0016192(vesicle-mediated transport)04144(Endocytosis)K12198NASNF7 domain containing protein, putative, expressed7.041.194.830.762.630.432.590.435.690.915.130.82
850chr1+178310180812LOC_Os01g01360.112503LOC_Os01g01360LOC_Os01g01360GO:0005215(transporter activity);GO:0005886(plasma membrane);GO:0006857(oligopeptide transport);GO:0016020(membrane);GO:0016021(integral component of membrane)NANANApeptide transporter PTR2, putative, expressed002.110.331.510.255.870.982.930.474.880.78
86pchr1-185073185970MSTRG.18.11898MSTRG.18LOC_Os01g01369NANANANANA16.042.7020.413.2015.952.6322.103.6919.093.0720.893.34
87jchr1-186138190825MSTRG.19.151550MSTRG.19LOC_Os01g01369NANANANANA0000000033.765.4300
88jchr1-186240188981MSTRG.19.222082MSTRG.19LOC_Os01g01369NANANANANA2.030.348.641.3510.811.78007.541.2111.071.77
890chr1-186246190846LOC_Os01g01369.141545MSTRG.19LOC_Os01g01369GO:0000247(C-8 sterol isomerase activity);GO:0005789(endoplasmic reticulum membrane);GO:0005886(plasma membrane);GO:0016021(integral component of membrane);GO:0016126(sterol biosynthetic process);GO:0047750(cholestenol delta-isomerase activity)00100(Steroid biosynthesis)K01824EC:5.3.3.53-beta-hydroxysteroid-Delta-isomerase, putative, expressed73.8112.4393.1714.5974.0912.2091.0315.2236.375.8542.986.87
900chr1+191036196287LOC_Os01g01380.163482MSTRG.20LOC_Os01g01380NANANANAexpressed protein3.890.654.220.664.750.782.600.444.120.665.400.86
910chr1+197647200803LOC_Os01g01390.142142MSTRG.23LOC_Os01g01390GO:0003674(molecular_function);GO:0005634(nucleus);GO:0005886(plasma membrane);GO:0008150(biological_process)NANANAexpressed protein89.8915.14104.3716.3450.398.29108.4218.1397.4415.67113.9418.23
920chr1+197647200803LOC_Os01g01390.252175MSTRG.23LOC_Os01g01390GO:0003674(molecular_function);GO:0005634(nucleus);GO:0005886(plasma membrane);GO:0008150(biological_process)NANANAexpressed protein0.060.010.090.010000009.651.54
930chr1+197647200803LOC_Os01g01390.352182MSTRG.23LOC_Os01g01390GO:0003674(molecular_function);GO:0005634(nucleus);GO:0005886(plasma membrane);GO:0008150(biological_process)NANANAexpressed protein11.581.950.300.0524.894.1042.827.1615.402.4830.644.90
940chr1+197647200803LOC_Os01g01390.442261MSTRG.23LOC_Os01g01390GO:0003674(molecular_function);GO:0005634(nucleus);GO:0005886(plasma membrane);GO:0008150(biological_process)NANANAexpressed protein009.011.4112.362.03008.731.4017.492.80
950chr1+197647200803LOC_Os01g01390.531054MSTRG.23LOC_Os01g01390GO:0003674(molecular_function);GO:0005634(nucleus);GO:0005886(plasma membrane);GO:0008150(biological_process)NANANAexpressed protein1.900.322.860.457.621.257.341.238.101.306.461.03
960chr1+201944206743LOC_Os01g01400.1122735MSTRG.26LOC_Os01g01400GO:0003674(molecular_function);GO:0005575(cellular_component);GO:0008150(biological_process)NANANAexpressed protein5.800.982.410.382.180.363.960.663.520.579.871.58
970chr1-205710209606LOC_Os01g01410.142489MSTRG.27LOC_Os01g01410GO:0004674(protein serine/threonine kinase activity);GO:0005886(plasma membrane)04626(Plant-pathogen interaction)K13430EC:2.7.11.1TKL_IRAK_C-LEC.1 - TKL_IRAK_C-LEC has homology to C-type lectin receptors, expressed23.483.9576.5911.9924.123.9773.2312.2457.949.3277.4412.39
980chr1-205710209606LOC_Os01g01410.242486MSTRG.27LOC_Os01g01410GO:0004674(protein serine/threonine kinase activity);GO:0005886(plasma membrane)04626(Plant-pathogen interaction)K13430EC:2.7.11.1TKL_IRAK_C-LEC.1 - TKL_IRAK_C-LEC has homology to C-type lectin receptors, expressed20.303.4232.015.0116.472.7138.376.4243.937.0665.8210.53
99jchr1-205711209582MSTRG.27.442528MSTRG.27LOC_Os01g01410NANANANANA000000001.090.1800
100jchr1-205711209600MSTRG.27.332613MSTRG.27LOC_Os01g01410NANANANANA12.102.047.541.187.391.2220.573.4416.782.705.000.80
101jchr1+209677214224MSTRG.28.172891MSTRG.28LOC_Os01g01420NANANANANA43.787.3710.711.6826.694.393.110.526.931.1125.934.15
1020chr1+209771214188LOC_Os01g01420.181657MSTRG.28LOC_Os01g01420GO:0005720(nuclear heterochromatin);GO:0010216(maintenance of DNA methylation);GO:0016567(protein ubiquitination);GO:0061630(ubiquitin protein ligase activity);GO:0090308(regulation of methylation-dependent chromatin silencing)NANANAexpressed protein11.441.9364.7110.1390.2614.8693.4415.62120.5419.38124.9219.98
1030chr1+209771214188LOC_Os01g01420.381653MSTRG.28LOC_Os01g01420GO:0005720(nuclear heterochromatin);GO:0010216(maintenance of DNA methylation);GO:0016567(protein ubiquitination);GO:0061630(ubiquitin protein ligase activity);GO:0090308(regulation of methylation-dependent chromatin silencing)NANANAexpressed protein13.572.2822.603.5443.157.1014.212.3812.211.9635.675.71
1040chr1+209903214229LOC_Os01g01420.271913MSTRG.28LOC_Os01g01420GO:0005720(nuclear heterochromatin);GO:0010216(maintenance of DNA methylation);GO:0016567(protein ubiquitination);GO:0061630(ubiquitin protein ligase activity);GO:0090308(regulation of methylation-dependent chromatin silencing)NANANAexpressed protein115.1419.3928.404.4530.234.9814.532.438.241.3340.576.49
1050chr1+216126217664LOC_Os01g01430.121425MSTRG.24LOC_Os01g01430GO:0003700(transcription factor activity, sequence-specific DNA binding);GO:0005634(nucleus);GO:0006351(transcription, DNA-templated);GO:0006355(regulation of transcription, DNA-templated);GO:0007275(multicellular organism development);GO:0009753(response to jasmonic acid);GO:0044212(transcription regulatory region DNA binding);GO:0045995(regulation of embryonic development);GO:0048317(seed morphogenesis);GO:0080060(integument development)00561(Glycerolipid metabolism)K01054EC:3.1.1.23No apical meristem protein, putative, expressed7.361.24000.830.140.210.030000

document location:

summary/4_transcript_expression/4_genes_fpkm_expression.xlsx

summary/4_transcript_expression/5_isoforms_fpkm_expression.xlsx


Class code typies and definitions:

class codeDescription
=/0Complete match (mRNA, lncRNA etc)
cContained
jPotentially novel isoform (fragment): at least one splice junction is hared with a reference transcript
eSingle exon transfrag overlapping a reference exon and at least 10 bp of a reference intron, indicating a possible pre-mRNA fragment.
iA transfrag falling entirely within a reference intron
oGeneric exonic overlap with a reference transcript
pPossible polymerase run-on fragment (within 2Kbases of a reference transcript)
rRepeat. Currently determined by looking at the soft-masked reference sequence and applied to transcripts where at least 50% of the are lower casebases
uUnknown, intergenic transcript
xExonic overlap with reference on the opposite strand
sAn intron of the transfrag overlaps a reference intron on the opposite strand (likely due to read mapping errors)
.(.tracking file only, indicates multiple classifications)

4.7 Differential expression analysis


Exploratory analysis, visualization and statistical modeling are the next steps after assembling and quantifying genes and transcripts from StringTie. The assembly of lncRNA-seq reads reconstructs the exon–intron structure of genes and their isoforms. R package ballgown takes both transcript and gene expression levels from StringTie and applies rigorous statistical methods to determine which transcripts and genes are differentially expressed between two or more experiments. Ballgown also includes plotting tools as part of the R/Bioconductor package that help visualize the results.


4.7.1 Profiling of differentially expressed genes/transcripts


Differentially expressed genes:

gene_idgene_nametranscript_idGOKEGGKO_ENTRYECDescriptionFPKM.Lo8_1FPKM.Lo8_2FPKM.Lo8_3FPKM.Lo7_1FPKM.Lo7_2FPKM.Lo7_3fclog2(fc)pvalqvalregulationsignificant
LOC_Os01g01090LOC_Os01g01090LOC_Os01g01090.1GO:0003755(peptidyl-prolyl cis-trans isomerase activity);GO:0005528(FK506 binding);GO:0005789(endoplasmic reticulum membrane);GO:0061077(chaperone-mediated protein folding)NANANAretrotransposon protein, putative, unclassified, expressed00.0200001.010.010.390.80upno
LOC_Os01g01110LOC_Os01g01110LOC_Os01g01110.1GO:0004672(protein kinase activity);GO:0004674(protein serine/threonine kinase activity);GO:0005524(ATP binding);GO:0005886(plasma membrane);GO:0006468(protein phosphorylation);GO:0007169(transmembrane receptor protein tyrosine kinase signaling pathway);GO:0016020(membrane);GO:0016021(integral component of membrane);GO:0016301(kinase activity)04075(Plant hormone signal transduction);04626(Plant-pathogen interaction)K13416EC:2.7.10.1;EC:2.7.11.1expressed protein0.59000001.270.340.380.80upno
LOC_Os01g01290LOC_Os01g01290LOC_Os01g01290.1GO:0003677(DNA binding);GO:0003700(transcription factor activity, sequence-specific DNA binding);GO:0005515(protein binding);GO:0005634(nucleus);GO:0005829(cytosol);GO:0006351(transcription, DNA-templated);GO:0006355(regulation of transcription, DNA-templated);GO:0009738(abscisic acid-activated signaling pathway);GO:0010468(regulation of gene expression);GO:0046982(protein heterodimerization activity);GO:0048586(regulation of long-day photoperiodism, flowering);GO:0051247(positive regulation of protein metabolic process);GO:2000905(negative regulation of starch metabolic process)NANANAhistone-like transcription factor and archaeal histone, putative, expressed00000.1100.98-0.030.710.93downno
LOC_Os01g01360LOC_Os01g01360LOC_Os01g01360.1GO:0005215(transporter activity);GO:0005886(plasma membrane);GO:0006857(oligopeptide transport);GO:0016020(membrane);GO:0016021(integral component of membrane)NANANApeptide transporter PTR2, putative, expressed0.980.470.7800.330.251.620.690.060.53upno
LOC_Os01g01470LOC_Os01g01470LOC_Os01g01470.1GO:0003677(DNA binding);GO:0003700(transcription factor activity, sequence-specific DNA binding);GO:0005634(nucleus);GO:0005737(cytoplasm);GO:0006351(transcription, DNA-templated);GO:0006355(regulation of transcription, DNA-templated);GO:0007275(multicellular organism development)03018(RNA degradation)K12581NAno apical meristem protein, putative, expressed0.080.0600001.070.100.100.57upno
LOC_Os01g01730LOC_Os01g01730LOC_Os01g01730.1NANANANAserine/arginine repetitive matrix protein 2, putative, expressed000.040001.000.001.001.00upno
LOC_Os01g01770LOC_Os01g01770LOC_Os01g01770.1GO:0003676(nucleic acid binding);GO:0004527(exonuclease activity);GO:0005622(intracellular);GO:0005634(nucleus);GO:0046872(metal ion binding)03008(Ribosome biogenesis in eukaryotes)K14570EC:3.1.-.-ZOS1-01 - C2H2 zinc finger protein, expressed0.270.0500.260.040.070.95-0.070.770.95downno
LOC_Os01g01840LOC_Os01g01840LOC_Os01g01840.1GO:0000977(RNA polymerase II regulatory region sequence-specific DNA binding);GO:0003700(transcription factor activity, sequence-specific DNA binding);GO:0006357(regulation of transcription from RNA polymerase II promoter);GO:0090575(RNA polymerase II transcription factor complex)NANANAhelix-loop-helix DNA-binding domain containing protein, expressed00.0500.020.0201.010.010.830.98upno
LOC_Os01g01950LOC_Os01g01950LOC_Os01g01950.1NANANANAexpressed protein00.21000.070.071.080.120.430.82upno
LOC_Os01g01980LOC_Os01g01980LOC_Os01g01980.1NANANANAexpressed protein0.16000001.080.110.380.80upno
LOC_Os01g01990LOC_Os01g01990LOC_Os01g01990.1NANANANAexpressed protein0000.29000.82-0.290.080.53downno
LOC_Os01g02110LOC_Os01g02110LOC_Os01g02110.1GO:0001046(core promoter sequence-specific DNA binding);GO:0001228(transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding);GO:0005634(nucleus);GO:0006366(transcription from RNA polymerase II promoter)04075(Plant hormone signal transduction);04712(Circadian rhythm - plant)K12126NAhelix-loop-helix DNA-binding domain containing protein, expressed000.0800.0301.00-0.010.911.00downno
LOC_Os01g02120LOC_Os01g02120LOC_Os01g02120.1GO:0005634(nucleus);GO:0005737(cytoplasm);GO:0008429(phosphatidylethanolamine binding);GO:0009737(response to abscisic acid);GO:0009738(abscisic acid-activated signaling pathway);GO:0010030(positive regulation of seed germination)04712(Circadian rhythm - plant)K16223NAosMFT2 MFT-Like2 homologous to Mother of FT and TFL1 gene; contains Pfam profile PF01161: Phosphatidylethanolamine-binding protein, expressed000.050001.000.001.001.00upno
LOC_Os01g02190LOC_Os01g02190LOC_Os01g02190.1GO:0005887(integral component of plasma membrane);GO:0009992(cellular water homeostasis);GO:0015250(water channel activity);GO:0015254(glycerol channel activity);GO:0034220(ion transmembrane transport)NANANAaquaporin protein, putative, expressed0.120.080.190.1700.170.97-0.040.740.94downno
LOC_Os01g02210LOC_Os01g02210LOC_Os01g02210.1GO:0003755(peptidyl-prolyl cis-trans isomerase activity);GO:0005528(FK506 binding);GO:0005789(endoplasmic reticulum membrane);GO:0061077(chaperone-mediated protein folding)NANANAretrotransposon protein, putative, Ty3-gypsy subclass000000.001.000.001.001.00upno
LOC_Os01g02250LOC_Os01g02250LOC_Os01g02250.1GO:0005515(protein binding);GO:0005524(ATP binding);GO:0005576(extracellular region);GO:0006952(defense response);GO:0043531(ADP binding)04626(Plant-pathogen interaction)K13457NARGA-1, putative, expressed000.06000.011.000.001.001.00upno
LOC_Os01g02310LOC_Os01g02310LOC_Os01g02310.1GO:0004674(protein serine/threonine kinase activity);GO:0005886(plasma membrane);GO:0006468(protein phosphorylation)04626(Plant-pathogen interaction)K13430EC:2.7.11.1expressed protein00000.0301.00-0.010.710.93downno
LOC_Os01g02370LOC_Os01g02370LOC_Os01g02370.1GO:0005886(plasma membrane)00520(Amino sugar and nucleotide sugar metabolism)K01183EC:3.2.1.14receptor-like kinase ARK1AS, putative, expressed0.0900.040.020.010.011.020.030.580.90upno
LOC_Os01g02710LOC_Os01g02710LOC_Os01g02710.1GO:0005886(plasma membrane)NANANALRk-type protein, putative, expressed0.0300.080.070.0300.96-0.060.120.60downno
LOC_Os01g02950LOC_Os01g02950LOC_Os01g02950.1NANANANAexpressed protein00.0800001.040.060.390.80upno
LOC_Os01g03260LOC_Os01g03260LOC_Os01g03260.1NANANANAexpressed protein000000.031.000.001.001.00upno
LOC_Os01g03270LOC_Os01g03270LOC_Os01g03270.1GO:0005634(nucleus);GO:0005737(cytoplasm);GO:0006915(apoptotic process);GO:0042787(protein ubiquitination involved in ubiquitin-dependent protein catabolic process);GO:0043161(proteasome-mediated ubiquitin-dependent protein catabolic process);GO:0061630(ubiquitin protein ligase activity)04120(Ubiquitin mediated proteolysis)K04506EC:2.3.2.27expressed protein0.490.270.200.120.100.021.230.300.150.63upno
LOC_Os01g03290LOC_Os01g03290LOC_Os01g03290.1GO:0005634(nucleus);GO:0005737(cytoplasm);GO:0006915(apoptotic process);GO:0042787(protein ubiquitination involved in ubiquitin-dependent protein catabolic process);GO:0043161(proteasome-mediated ubiquitin-dependent protein catabolic process);GO:0061630(ubiquitin protein ligase activity)04120(Ubiquitin mediated proteolysis)K04506EC:2.3.2.27seven in absentia protein family domain containing protein, expressed00.1700001.080.110.390.80upno
LOC_Os01g03300LOC_Os01g03300LOC_Os01g03300.1GO:0005634(nucleus);GO:0005737(cytoplasm);GO:0006915(apoptotic process);GO:0042787(protein ubiquitination involved in ubiquitin-dependent protein catabolic process);GO:0043161(proteasome-mediated ubiquitin-dependent protein catabolic process);GO:0061630(ubiquitin protein ligase activity)04120(Ubiquitin mediated proteolysis)K04506EC:2.3.2.27seven in absentia protein family protein, expressed0000.020.060.170.97-0.040.610.91downno
LOC_Os01g03320LOC_Os01g03320LOC_Os01g03320.1NANANANABBTI2 - Bowman-Birk type bran trypsin inhibitor precursor, expressed0.870.200.700.740.810.780.86-0.220.510.86downno
LOC_Os01g03380LOC_Os01g03380LOC_Os01g03380.1NANANANABBTI6 - Bowman-Birk type bran trypsin inhibitor precursor, putative, expressed00.060.12000.321.030.040.840.98upno
LOC_Os01g03429LOC_Os01g03429LOC_Os01g03429.1GO:0005634(nucleus);GO:0005737(cytoplasm);GO:0006915(apoptotic process);GO:0042787(protein ubiquitination involved in ubiquitin-dependent protein catabolic process);GO:0043161(proteasome-mediated ubiquitin-dependent protein catabolic process);GO:0061630(ubiquitin protein ligase activity)04120(Ubiquitin mediated proteolysis)K04506EC:2.3.2.27expressed protein0.0600.120.100.0400.95-0.080.160.65downno
LOC_Os01g03464LOC_Os01g03464LOC_Os01g03464.1GO:0005634(nucleus);GO:0005737(cytoplasm);GO:0006915(apoptotic process);GO:0042787(protein ubiquitination involved in ubiquitin-dependent protein catabolic process);GO:0043161(proteasome-mediated ubiquitin-dependent protein catabolic process);GO:0061630(ubiquitin protein ligase activity)04120(Ubiquitin mediated proteolysis)K04506EC:2.3.2.27expressed protein000.0300.0400.99-0.010.770.95downno
LOC_Os01g03620LOC_Os01g03620LOC_Os01g03620.1GO:0005507(copper ion binding);GO:0016722(oxidoreductase activity, oxidizing metal ions)NANANAmulticopper oxidase domain containing protein, expressed0.110.140.470.380.280.490.82-0.280.260.76downno
LOC_Os01g03670LOC_Os01g03670LOC_Os01g03670.1GO:0003824(catalytic activity);GO:0005829(cytosol);GO:0010584(pollen exine formation);GO:0016491(oxidoreductase activity);GO:0050662(coenzyme binding);GO:0055114(oxidation-reduction process);GO:0080110(sporopollenin biosynthetic process)00940(Phenylpropanoid biosynthesis)K09753EC:1.2.1.44dihydroflavonol-4-reductase, putative, expressed0.070.040.040.050.150.080.99-0.020.750.95downno
LOC_Os01g03840LOC_Os01g03840LOC_Os01g03840.1GO:0003700(transcription factor activity, sequence-specific DNA binding);GO:0005634(nucleus);GO:0008285(negative regulation of cell proliferation);GO:0010094(specification of carpel identity);GO:0010097(specification of stamen identity);GO:0043565(sequence-specific DNA binding);GO:0044212(transcription regulatory region DNA binding);GO:0048437(floral organ development)03410(Base excision repair)K10773EC:4.2.99.18ZOS1-02 - C2H2 zinc finger protein, expressed0.710.640.190.130.140.481.480.570.060.53upno
LOC_Os01g03890LOC_Os01g03890LOC_Os01g03890.1GO:0009739(response to gibberellin)NANANADUF260 domain containing protein, putative, expressed00.020.070.000.2200.97-0.040.810.97downno
LOC_Os01g03900LOC_Os01g03900LOC_Os01g03900.1NANANANAexpressed protein000001.581.000.001.001.00upno
LOC_Os01g03930LOC_Os01g03930LOC_Os01g03930.1NANANANAexpressed protein00000.4700.93-0.110.710.93downno
LOC_Os01g03960LOC_Os01g03960LOC_Os01g03960.1NANANANAexpressed protein0.12000001.060.080.380.80upno
LOC_Os01g04005LOC_Os01g04005LOC_Os01g04005.1NANANANAexpressed protein0000.010.0100.99-0.010.040.52downno
LOC_Os01g04090LOC_Os01g04090LOC_Os01g04090.1NANANANAexpressed protein0.430.430.510.370.630.601.010.010.901.00upno
LOC_Os01g04210LOC_Os01g04210LOC_Os01g04210.1GO:0008375(acetylglucosaminyltransferase activity);GO:0016021(integral component of membrane);GO:0016757(transferase activity, transferring glycosyl groups)04144(Endocytosis)K12471NADNA binding protein, putative, expressed0.210.120.250.380.0600.89-0.170.180.67downno
LOC_Os01g04220LOC_Os01g04220LOC_Os01g04220.1GO:0008375(acetylglucosaminyltransferase activity);GO:0016021(integral component of membrane);GO:0016757(transferase activity, transferring glycosyl groups)04144(Endocytosis)K12471NADNA binding protein, putative, expressed0.040.200.190.060.160.121.030.050.710.93upno
LOC_Os01g04250LOC_Os01g04250LOC_Os01g04250.1GO:0007165(signal transduction);GO:0016020(membrane)NANANAexpressed protein0.560000.210.261.210.270.410.81upno
LOC_Os01g04540LOC_Os01g04540LOC_Os01g04540.1NANANANAserine/threonine-protein kinase At1g18390 precursor, putative, expressed0.120.400.040.280.130.551.000.000.991.00upno
LOC_Os01g04550LOC_Os01g04550LOC_Os01g04550.1GO:0004674(protein serine/threonine kinase activity);GO:0005524(ATP binding);GO:0005886(plasma membrane);GO:0006468(protein phosphorylation);GO:0016021(integral component of membrane);GO:0016301(kinase activity);GO:0016310(phosphorylation)00520(Amino sugar and nucleotide sugar metabolism)K01183EC:3.2.1.14expressed protein0.170.210.350.220.330.310.96-0.070.590.90downno
LOC_Os01g04560LOC_Os01g04560LOC_Os01g04560.1GO:0004674(protein serine/threonine kinase activity);GO:0005886(plasma membrane);GO:0007166(cell surface receptor signaling pathway)NANANAexpressed protein0000.050.200.020.93-0.110.390.80downno
LOC_Os01g04640LOC_Os01g04640LOC_Os01g04640.1GO:0003674(molecular_function);GO:0005739(mitochondrion);GO:0005783(endoplasmic reticulum);GO:0005789(endoplasmic reticulum membrane);GO:0008150(biological_process);GO:0016021(integral component of membrane)NANANAreticulon domain containing protein, putative, expressed0.850.490.480.510.520.231.100.130.590.90upno
LOC_Os01g04699LOC_Os01g04699LOC_Os01g04699.1NANANANAexpressed protein0.210.200.130.150.2401.030.040.800.96upno
LOC_Os01g04750LOC_Os01g04750LOC_Os01g04750.1GO:0003677(DNA binding);GO:0003700(transcription factor activity, sequence-specific DNA binding);GO:0005634(nucleus);GO:0006351(transcription, DNA-templated);GO:0009873(ethylene-activated signaling pathway);GO:0045892(negative regulation of transcription, DNA-templated)NANANAB3 DNA binding domain containing protein, expressed0.0300.090001.020.020.660.93upno
LOC_Os01g04800LOC_Os01g04800LOC_Os01g04800.1GO:0003677(DNA binding);GO:0003700(transcription factor activity, sequence-specific DNA binding);GO:0005634(nucleus);GO:0006351(transcription, DNA-templated);GO:0006355(regulation of transcription, DNA-templated);GO:0009873(ethylene-activated signaling pathway);GO:0048573(photoperiodism, flowering)04075(Plant hormone signal transduction)K14486NAB3 DNA binding domain containing protein, expressed0.250.230.630.770.470.260.73-0.460.010.40downno
LOC_Os01g05050LOC_Os01g05050LOC_Os01g05050.1GO:0003674(molecular_function);GO:0005739(mitochondrion);GO:0005759(mitochondrial matrix);GO:0008150(biological_process)NANANAmitochondrial glycoprotein, putative, expressed000.060001.000.001.001.00upno
LOC_Os01g05120LOC_Os01g05120LOC_Os01g05120.1GO:0003674(molecular_function);GO:0005829(cytosol);GO:0006511(ubiquitin-dependent protein catabolic process);GO:0009507(chloroplast)04141(Protein processing in endoplasmic reticulum)K14016NAubiquitin fusion degradation protein, putative, expressed0.120.130.180.21000.97-0.050.450.83downno
LOC_Os01g05340LOC_Os01g05340LOC_Os01g05340.1NANANANAexpressed protein0.190.190.080.170.080.181.040.050.620.91upno
LOC_Os01g05360LOC_Os01g05360LOC_Os01g05360.1GO:0009834(plant-type secondary cell wall biogenesis);GO:0010417(glucuronoxylan biosynthetic process);GO:0042285(xylosyltransferase activity)NANANAexpressed protein00.2500001.110.160.390.80upno
LOC_Os01g05380LOC_Os01g05380LOC_Os01g05380.1NANANANAexpressed protein000000.021.000.001.001.00upno
LOC_Os01g05400LOC_Os01g05400LOC_Os01g05400.1GO:0009834(plant-type secondary cell wall biogenesis);GO:0010417(glucuronoxylan biosynthetic process);GO:0042285(xylosyltransferase activity)NANANAexpressed protein000000.051.000.001.001.00upno
LOC_Os01g05440LOC_Os01g05440LOC_Os01g05440.1GO:0004526(ribonuclease P activity);GO:0005655(nucleolar ribonuclease P complex);GO:0090502(RNA phosphodiester bond hydrolysis, endonucleolytic)NANANAXH domain containing protein, expressed0.170.140.110.090.340.021.010.020.921.00upno
LOC_Os01g05450LOC_Os01g05450LOC_Os01g05450.1NANANANAexpressed protein0.240.1300.450.130.120.85-0.230.370.80downno
LOC_Os01g05510LOC_Os01g05510LOC_Os01g05510.4NANANANAexpressed protein000.020001.000.001.001.00upno
LOC_Os01g05530LOC_Os01g05530LOC_Os01g05530.1GO:0000027(ribosomal large subunit assembly);GO:0003723(RNA binding);GO:0005730(nucleolus)NANANAexpressed protein000.060001.000.001.001.00upno
LOC_Os01g05580LOC_Os01g05580LOC_Os01g05580.1NANANANAExo70 exocyst complex subunit family, putative, expressed0.05000001.020.030.380.80upno
LOC_Os01g05585LOC_Os01g05585LOC_Os01g05585.1NANANANAmetallothionein, putative, expressed0.3700.2500.1001.150.210.420.81upno
LOC_Os01g05600LOC_Os01g05600LOC_Os01g05600.1GO:0005515(protein binding);GO:0005524(ATP binding);GO:0005576(extracellular region);GO:0006952(defense response);GO:0043531(ADP binding)04626(Plant-pathogen interaction)K13457NANBS-LRR disease resistance protein, putative, expressed0.42000001.200.260.380.80upno
LOC_Os01g06030LOC_Os01g06030LOC_Os01g06030.1GO:0005634(nucleus)00350(Tyrosine metabolism);00950(Isoquinoline alkaloid biosynthesis)K01592EC:4.1.1.25expressed protein0.07000001.030.050.380.80upno
LOC_Os01g06040LOC_Os01g06040LOC_Os01g06040.1NA00350(Tyrosine metabolism);00950(Isoquinoline alkaloid biosynthesis)K01592EC:4.1.1.25expressed protein000.1000.0900.98-0.030.810.97downno
LOC_Os01g06060LOC_Os01g06060LOC_Os01g06060.1GO:0005634(nucleus);GO:0008152(metabolic process);GO:0052689(carboxylic ester hydrolase activity)04075(Plant hormone signal transduction)K14493EC:3.-.-.-gibberellin receptor GID1L2, putative, expressed0000.040.040.040.96-0.060.000.11downno
LOC_Os01g06070LOC_Os01g06070LOC_Os01g06070.1NANANANAexpressed protein0000.110.0600.91-0.140.050.53downno
LOC_Os01g06120LOC_Os01g06120LOC_Os01g06120.1NANANANAexpressed protein0000.03000.97-0.040.080.53downno
LOC_Os01g06150LOC_Os01g06150LOC_Os01g06150.1GO:0005634(nucleus)NANANAexpressed protein0.630.560.820.700.290.920.99-0.010.971.00downno
LOC_Os01g06210LOC_Os01g06210LOC_Os01g06210.1GO:0005634(nucleus);GO:0008152(metabolic process);GO:0052689(carboxylic ester hydrolase activity)04075(Plant hormone signal transduction)K14493EC:3.-.-.-gibberellin receptor GID1L2, putative, expressed0.00000001.000.000.380.80upno
LOC_Os01g06220LOC_Os01g06220LOC_Os01g06220.1GO:0005634(nucleus);GO:0008152(metabolic process);GO:0052689(carboxylic ester hydrolase activity)04075(Plant hormone signal transduction)K14493EC:3.-.-.-gibberellin receptor GID1L2, putative, expressed0.070.120.100.1800.040.96-0.060.500.86downno
LOC_Os01g06240LOC_Os01g06240LOC_Os01g06240.1GO:0004674(protein serine/threonine kinase activity);GO:0005886(plasma membrane);GO:0006468(protein phosphorylation)00260(Glycine, serine and threonine metabolism);00350(Tyrosine metabolism);00360(Phenylalanine metabolism);00410(beta-Alanine metabolism);00950(Isoquinoline alkaloid biosynthesis);00960(Tropane, piperidine and pyridine alkaloid biosynthesis)K00276EC:1.4.3.21protein kinase, putative, expressed00.0600.080.0600.96-0.070.360.80downno
LOC_Os01g06370LOC_Os01g06370LOC_Os01g06370.1NANANANAexpressed protein00.08000.3400.98-0.030.911.00downno
LOC_Os01g06390LOC_Os01g06390LOC_Os01g06390.1NANANANAexpressed protein000.080001.000.001.001.00upno
LOC_Os01g06400LOC_Os01g06400LOC_Os01g06400.1NANANANAexpressed protein0000.030.1100.95-0.070.380.80downno
LOC_Os01g06420LOC_Os01g06420LOC_Os01g06420.1NANANANAexpressed protein0.240.460.1500.1501.310.380.100.57upno
LOC_Os01g06430LOC_Os01g06430LOC_Os01g06430.1NANANANAexpressed protein0.250.0600001.150.200.230.73upno
LOC_Os01g06540LOC_Os01g06540LOC_Os01g06540.1GO:0003677(DNA binding);GO:0005634(nucleus);GO:0005829(cytosol);GO:0006351(transcription, DNA-templated);GO:0006355(regulation of transcription, DNA-templated);GO:0008270(zinc ion binding);GO:0016036(cellular response to phosphate starvation);GO:0016568(chromatin modification);GO:0035064(methylated histone binding);GO:0042393(histone binding);GO:0048767(root hair elongation);GO:0055065(metal ion homeostasis)NANANAPHD finger protein, putative, expressed00.2600.170.1000.97-0.040.850.98downno
LOC_Os01g06670LOC_Os01g06670LOC_Os01g06670.1GO:0006952(defense response);GO:0007165(signal transduction);GO:0016021(integral component of membrane);GO:0016301(kinase activity)04626(Plant-pathogen interaction)K13466NAhcr2-5D, putative, expressed0.02000001.010.010.380.80upno
LOC_Os01g06720LOC_Os01g06720LOC_Os01g06720.1GO:0006952(defense response);GO:0007165(signal transduction);GO:0016021(integral component of membrane);GO:0016301(kinase activity)04626(Plant-pathogen interaction)K13420EC:2.7.11.1disease resistance protein SlVe2 precursor, putative, expressed0.040.020.010.1400.090.93-0.110.260.76downno
LOC_Os01g06750LOC_Os01g06750LOC_Os01g06750.1GO:0005886(plasma membrane);GO:0006952(defense response);GO:0007165(signal transduction);GO:0016301(kinase activity)04075(Plant hormone signal transduction)K13415EC:2.7.10.1;EC:2.7.11.1verticillium wilt disease resistance protein precursor, putative, expressed00.160.140.060.050.111.020.020.840.98upno
LOC_Os01g06790LOC_Os01g06790LOC_Os01g06790.1GO:0006952(defense response);GO:0007165(signal transduction);GO:0016021(integral component of membrane);GO:0016301(kinase activity)04626(Plant-pathogen interaction)K13420EC:2.7.11.1disease resistance protein, putative, expressed0.0300.020.020.080.030.98-0.030.590.90downno
LOC_Os01g06820LOC_Os01g06820LOC_Os01g06820.1GO:0006952(defense response);GO:0007165(signal transduction);GO:0016021(integral component of membrane);GO:0016301(kinase activity)04626(Plant-pathogen interaction)K13420EC:2.7.11.1hcr2-0B, putative, expressed0.100.130.050.1500.100.99-0.010.931.00downno
LOC_Os01g06836LOC_Os01g06836LOC_Os01g06836.1GO:0005886(plasma membrane);GO:0006952(defense response);GO:0007165(signal transduction);GO:0016301(kinase activity)04626(Plant-pathogen interaction)K13466NAdisease resistance protein SlVe2 precursor, putative, expressed0.050.0300.100.0500.96-0.060.360.80downno
LOC_Os01g06870LOC_Os01g06870LOC_Os01g06870.1GO:0006952(defense response);GO:0007165(signal transduction);GO:0016021(integral component of membrane)04626(Plant-pathogen interaction)K13420EC:2.7.11.1resistance protein SlVe1 precursor, putative, expressed000.0500.0300.99-0.010.840.98downno
LOC_Os01g06920LOC_Os01g06920LOC_Os01g06920.1GO:0006952(defense response);GO:0007165(signal transduction);GO:0016021(integral component of membrane);GO:0016301(kinase activity)04626(Plant-pathogen interaction)K13466NAresistance protein SlVe1 precursor, putative, expressed0.440.510.540.160.3201.240.310.170.66upno
LOC_Os01g07020LOC_Os01g07020LOC_Os01g07020.1GO:0005887(integral component of plasma membrane);GO:0015179(L-amino acid transmembrane transporter activity);GO:0015297(antiporter activity)NANANAPOEI39 - Pollen Ole e I allergen and extensin family protein precursor, putative, expressed0000.0900.040.93-0.100.110.58downno
LOC_Os01g07030LOC_Os01g07030LOC_Os01g07030.1GO:0005887(integral component of plasma membrane);GO:0015179(L-amino acid transmembrane transporter activity);GO:0015297(antiporter activity)NANANAPOEI40 - Pollen Ole e I allergen and extensin family protein precursor, expressed0.090.180.320.030.120.211.080.110.530.87upno
LOC_Os01g07040LOC_Os01g07040LOC_Os01g07040.1GO:0005887(integral component of plasma membrane);GO:0015179(L-amino acid transmembrane transporter activity);GO:0015297(antiporter activity)NANANAPOEI41 - Pollen Ole e I allergen and extensin family protein precursor, expressed000.120001.000.001.001.00upno
LOC_Os01g07150LOC_Os01g07150LOC_Os01g07150.1GO:0003674(molecular_function);GO:0008150(biological_process)NANANAexpressed protein00.0800001.040.050.390.80upno
LOC_Os01g07160LOC_Os01g07160LOC_Os01g07160.1GO:0004842(ubiquitin-protein transferase activity);GO:0019005(SCF ubiquitin ligase complex);GO:0031146(SCF-dependent proteasomal ubiquitin-dependent protein catabolic process)NANANAOsFBX3 - F-box domain containing protein, expressed00.0400.210.2800.83-0.270.190.68downno
LOC_Os01g07180LOC_Os01g07180LOC_Os01g07180.1NANANANAexpressed protein0.1500.10000.101.070.100.430.81upno
LOC_Os01g07364LOC_Os01g07364LOC_Os01g07364.1NANANANAexpressed protein000.110001.000.001.001.00upno
LOC_Os01g07430LOC_Os01g07430LOC_Os01g07430.1GO:0003677(DNA binding);GO:0003700(transcription factor activity, sequence-specific DNA binding);GO:0005634(nucleus);GO:0006355(regulation of transcription, DNA-templated);GO:0009651(response to salt stress);GO:0009723(response to ethylene);GO:0009737(response to abscisic acid);GO:0009753(response to jasmonic acid)00190(Oxidative phosphorylation);00860(Porphyrin and chlorophyll metabolism)K02259NAmyb-like DNA-binding domain containing protein, putative, expressed0.070.350.290.030.060.051.150.210.280.78upno
LOC_Os01g07450LOC_Os01g07450LOC_Os01g07450.1GO:0003677(DNA binding);GO:0005634(nucleus);GO:0006351(transcription, DNA-templated);GO:0006355(regulation of transcription, DNA-templated);GO:0045892(negative regulation of transcription, DNA-templated)00190(Oxidative phosphorylation);00860(Porphyrin and chlorophyll metabolism)K02259NAMYB family transcription factor, putative, expressed0.060.060.030.1300.130.96-0.060.590.90downno
LOC_Os01g07460LOC_Os01g07460LOC_Os01g07460.1NANANANAconserved hypothetical protein0.14000001.070.100.380.80upno
LOC_Os01g07550LOC_Os01g07550LOC_Os01g07550.1NANANANAhypothetical protein000.060001.000.001.001.00upno
LOC_Os01g07640LOC_Os01g07640LOC_Os01g07640.1GO:0005575(cellular_component);GO:0008150(biological_process)04075(Plant hormone signal transduction)K14500EC:2.7.11.1ankyrin, putative, expressed0.210.120.150.170.040.071.020.030.710.93upno
LOC_Os01g07660LOC_Os01g07660LOC_Os01g07660.1NANANANAexpressed protein00000.0500.99-0.010.710.93downno
LOC_Os01g07710LOC_Os01g07710LOC_Os01g07710.1NANANANAexpressed protein0.040.230.14000.061.130.180.210.71upno
LOC_Os01g07770LOC_Os01g07770LOC_Os01g07770.1GO:0004601(peroxidase activity);GO:0009505(plant-type cell wall);GO:0009664(plant-type cell wall organization)00940(Phenylpropanoid biosynthesis)K00430EC:1.11.1.7peroxidase precursor, putative, expressed0.270.450.630.380.690.560.94-0.080.710.93downno
LOC_Os01g08070LOC_Os01g08070LOC_Os01g08070.1GO:0000147(actin cortical patch assembly);GO:0005884(actin filament);GO:0006897(endocytosis);GO:0008360(regulation of cell shape);GO:0030048(actin filament-based movement);GO:0030479(actin cortical patch);GO:0051015(actin filament binding);GO:0051127(positive regulation of actin nucleation);GO:0051666(actin cortical patch localization)NANANAtransposon protein, putative, CACTA, En/Spm sub-class, expressed00000.0201.00-0.000.710.93downno


Differentially expressed transcripts:

t_idclass_codechrstrandstartendt_namenum_exonslengthgene_idgene_nameGOKEGGKO_ENTRYECDescriptioncov.Lo8_1FPKM.Lo8_1cov.Lo8_2FPKM.Lo8_2cov.Lo8_3FPKM.Lo8_3cov.Lo7_1FPKM.Lo7_1cov.Lo7_2FPKM.Lo7_2cov.Lo7_3FPKM.Lo7_3fclog2(fc)pvalqvalregulationsignificant
10chr1+290310817LOC_Os01g01010.1123017MSTRG.3LOC_Os01g01010GO:0005096(GTPase activator activity);GO:0005622(intracellular);GO:0006886(intracellular protein transport);GO:0012505(endomembrane system);GO:0017137(Rab GTPase binding);GO:0031338(regulation of vesicle fusion)03040(Spliceosome)K12840NATBC domain containing protein, expressed22.483.7632.345.2030.974.9515.392.5933.215.2022.183.651.180.240.470.78upno
2jchr1+292610822MSTRG.3.2122986MSTRG.3LOC_Os01g01010NANANANANA20.503.4326.734.3038.236.1236.846.2026.394.1319.533.211.080.110.400.78upno
3jchr1+293810822MSTRG.3.3122923MSTRG.3LOC_Os01g01010NANANANANA0.530.0900005.830.981.410.220.590.100.76-0.390.230.76downno
4jchr1+296810811MSTRG.3.4132857MSTRG.3LOC_Os01g01010NANANANANA00000000000.870.140.95-0.070.370.78downno
50chr1+298410562LOC_Os01g01010.2122218MSTRG.3LOC_Os01g01010GO:0005096(GTPase activator activity);GO:0005622(intracellular);GO:0006886(intracellular protein transport);GO:0012505(endomembrane system);GO:0017137(Rab GTPase binding);GO:0031338(regulation of vesicle fusion)03040(Spliceosome)K12840NATBC domain containing protein, expressed0.010.00000.070.010.090.020.050.010.000.001.00-0.000.450.78downno
6jchr1+1108620323MSTRG.4.1136352MSTRG.4LOC_Os01g01040NANANANANA0000000031.284.90000.53-0.900.420.78downno
7jchr1+1108620323MSTRG.4.2136775MSTRG.4LOC_Os01g01040NANANANANA2.240.37004.060.65005.510.866.010.990.84-0.260.600.84downno
80chr1+1121812435LOC_Os01g01019.121127MSTRG.4LOC_Os01g01019NANANANAexpressed protein0000000.750.13000.400.070.95-0.080.210.74downno
9jchr1+1262720377MSTRG.4.4115478MSTRG.4LOC_Os01g01040NANANANANA7.621.27000020.383.43009.411.550.60-0.750.450.78downno
10jchr1+1262720377MSTRG.4.5125285MSTRG.4LOC_Os01g01040NANANANANA21.453.590017.342.7716.702.8100001.840.880.380.78upno
110chr1+1264815915LOC_Os01g01030.152464MSTRG.4LOC_Os01g01030GO:0005507(copper ion binding);GO:0009505(plant-type cell wall);GO:0009506(plasmodesma);GO:0016722(oxidoreductase activity, oxidizing metal ions)00053(Ascorbate and aldarate metabolism)K00423EC:1.10.3.3monocopper oxidase, putative, expressed00004.370.70001.180.18001.170.220.410.78upno
12jchr1+1265520323MSTRG.4.7105494MSTRG.4LOC_Os01g01040NANANANANA2.190.3717.792.8632.295.164.530.760.570.09002.841.500.060.66upno
13jchr1+1602420323MSTRG.4.892685MSTRG.4LOC_Os01g01040NANANANANA4.490.758.141.31002.260.380.820.13001.330.410.370.78upno
14jchr1+1602920320MSTRG.4.992674MSTRG.4LOC_Os01g01040NANANANANA4.060.689.141.47003.050.511.670.26001.260.330.510.80upno
150chr1+1629218304LOC_Os01g01040.431524MSTRG.4LOC_Os01g01040GO:0003674(molecular_function);GO:0005739(mitochondrion);GO:0008150(biological_process);GO:0016021(integral component of membrane)NANANAexpressed protein00000.200.030.060.012.110.330.710.120.88-0.190.240.77downno
160chr1+1629220323LOC_Os01g01040.182508MSTRG.4LOC_Os01g01040GO:0003674(molecular_function);GO:0005739(mitochondrion);GO:0008150(biological_process);GO:0016021(integral component of membrane)NANANAexpressed protein2.700.458.961.440.400.061.650.288.441.3211.141.830.71-0.500.190.74downno
170chr1+1632120323LOC_Os01g01040.282482MSTRG.4LOC_Os01g01040GO:0003674(molecular_function);GO:0005739(mitochondrion);GO:0008150(biological_process);GO:0016021(integral component of membrane)NANANAexpressed protein3.070.518.021.290.290.051.150.194.180.6519.033.130.70-0.520.350.78downno
180chr1+1632120323LOC_Os01g01040.372583MSTRG.4LOC_Os01g01040GO:0003674(molecular_function);GO:0005739(mitochondrion);GO:0008150(biological_process);GO:0016021(integral component of membrane)NANANAexpressed protein3.290.559.611.542.790.451.160.202.920.4619.453.200.86-0.220.730.90downno
190chr1+2284126971LOC_Os01g01050.162039MSTRG.1LOC_Os01g01050NANANANAR3H domain containing protein, expressed6.471.088.231.320.850.1416.812.833.310.526.551.080.77-0.390.560.82downno
200chr1+2284126971LOC_Os01g01050.261996MSTRG.1LOC_Os01g01050NANANANAR3H domain containing protein, expressed21.033.5213.282.1427.274.3618.743.1611.281.7722.153.651.150.210.580.83upno
21jchr1+2285026978MSTRG.1.352327MSTRG.1LOC_Os01g01050NANANANANA2.090.3512.001.9326.444.2312.342.085.700.8913.122.161.120.170.770.92upno
220chr1+2713628651LOC_Os01g01060.14920MSTRG.2LOC_Os01g01060GO:0000028(ribosomal small subunit assembly);GO:0003729(mRNA binding);GO:0003735(structural constituent of ribosome);GO:0006412(translation);GO:0019843(rRNA binding);GO:0022627(cytosolic small ribosomal subunit)03010(Ribosome)K02989NA40S ribosomal protein S5, putative, expressed893.89149.45729.34117.29878.38140.51535.2790.14624.3697.77532.5887.671.470.560.020.55upno
23jchr1+2977334495MSTRG.5.1132115MSTRG.5LOC_Os01g01070NANANANANA23.843.9910.421.6810.811.738.021.358.851.3926.324.331.010.020.970.99upno
24jchr1+2978934480MSTRG.5.2121994MSTRG.5LOC_Os01g01070NANANANANA6.521.098.141.3113.222.129.221.5510.441.647.971.311.020.020.870.96upno
250chr1+2981833943LOC_Os01g01070.3131369MSTRG.5LOC_Os01g01070GO:0003674(molecular_function);GO:0005739(mitochondrion);GO:0008150(biological_process);GO:0016021(integral component of membrane);GO:0031307(integral component of mitochondrial outer membrane)NANANAexpressed protein8.341.398.651.395.190.838.291.4010.251.606.701.100.91-0.130.460.78downno
260chr1+2981834493LOC_Os01g01070.1141568MSTRG.5LOC_Os01g01070GO:0003674(molecular_function);GO:0005739(mitochondrion);GO:0008150(biological_process);GO:0016021(integral component of membrane);GO:0031307(integral component of mitochondrial outer membrane)NANANAexpressed protein2.770.465.620.903.870.625.530.936.781.060.550.091.030.040.910.97upno
270chr1+2981834493LOC_Os01g01070.2141562MSTRG.5LOC_Os01g01070GO:0003674(molecular_function);GO:0005739(mitochondrion);GO:0008150(biological_process);GO:0016021(integral component of membrane);GO:0031307(integral component of mitochondrial outer membrane)NANANAexpressed protein42.567.1246.447.4759.999.6078.9113.2969.3510.8642.817.050.85-0.240.270.78downno
28jchr1+3539241235MSTRG.6.2132562MSTRG.6LOC_Os01g01080NANANANANA003.230.524.660.753.700.6220.493.214.220.690.61-0.700.320.78downno
29jchr1+3539241235MSTRG.6.3132814MSTRG.6LOC_Os01g01080NANANANANA1.390.231.400.221.510.240.980.173.290.522.220.360.91-0.140.310.78downno
30jchr1+3539241253MSTRG.6.1142324MSTRG.6LOC_Os01g01080NANANANANA12.152.0348.937.8712.562.019.091.5318.912.9645.717.520.90-0.150.830.94downno
31jchr1+3545041253MSTRG.6.4142266MSTRG.6LOC_Os01g01080NANANANANA1.640.278.751.418.541.3712.742.1526.784.193.610.590.68-0.560.440.78downno
32jchr1+3557941188MSTRG.6.5142231MSTRG.6LOC_Os01g01080NANANANANA29.444.9218.182.9242.956.8729.604.9817.972.8118.913.111.320.400.180.74upno
33jchr1+3557941188MSTRG.6.6122466MSTRG.6LOC_Os01g01080NANANANANA8.521.421.370.229.941.591.650.280.650.102.130.351.630.710.160.74upno
340chr1+3558141180LOC_Os01g01080.1151955MSTRG.6LOC_Os01g01080GO:0005783(endoplasmic reticulum);GO:0005789(endoplasmic reticulum membrane);GO:0006520(cellular amino acid metabolic process);GO:0008117(sphinganine-1-phosphate aldolase activity);GO:0016020(membrane);GO:0016021(integral component of membrane);GO:0016831(carboxy-lyase activity);GO:0019752(carboxylic acid metabolic process);GO:0030149(sphingolipid catabolic process);GO:0030170(pyridoxal phosphate binding)00600(Sphingolipid metabolism)K01634EC:4.1.2.27decarboxylase, putative, expressed174.3729.15114.3618.3990.6114.49214.3136.09122.4419.17162.2126.700.75-0.410.420.78downno
350chr1+3558141180LOC_Os01g01080.2151976MSTRG.6LOC_Os01g01080GO:0005783(endoplasmic reticulum);GO:0005789(endoplasmic reticulum membrane);GO:0006520(cellular amino acid metabolic process);GO:0008117(sphinganine-1-phosphate aldolase activity);GO:0016020(membrane);GO:0016021(integral component of membrane);GO:0016831(carboxy-lyase activity);GO:0019752(carboxylic acid metabolic process);GO:0030149(sphingolipid catabolic process);GO:0030170(pyridoxal phosphate binding)00600(Sphingolipid metabolism)K01634EC:4.1.2.27decarboxylase, putative, expressed2.120.350.270.048.331.330.320.05000.260.041.510.600.110.74upno
360chr1+3558141180LOC_Os01g01080.3142042MSTRG.6LOC_Os01g01080GO:0005783(endoplasmic reticulum);GO:0005789(endoplasmic reticulum membrane);GO:0006520(cellular amino acid metabolic process);GO:0008117(sphinganine-1-phosphate aldolase activity);GO:0016020(membrane);GO:0016021(integral component of membrane);GO:0016831(carboxy-lyase activity);GO:0019752(carboxylic acid metabolic process);GO:0030149(sphingolipid catabolic process);GO:0030170(pyridoxal phosphate binding)00600(Sphingolipid metabolism)K01634EC:4.1.2.27decarboxylase, putative, expressed5.930.990.560.092.430.391.680.289.201.440.610.100.94-0.080.880.96downno
370chr1+4459547526LOC_Os01g01090.182409LOC_Os01g01090LOC_Os01g01090GO:0003755(peptidyl-prolyl cis-trans isomerase activity);GO:0005528(FK506 binding);GO:0005789(endoplasmic reticulum membrane);GO:0061077(chaperone-mediated protein folding)NANANAretrotransposon protein, putative, unclassified, expressed000.130.02000000001.010.010.470.78upno
380chr1+5708257972LOC_Os01g01110.11891LOC_Os01g01110LOC_Os01g01110GO:0004672(protein kinase activity);GO:0004674(protein serine/threonine kinase activity);GO:0005524(ATP binding);GO:0005886(plasma membrane);GO:0006468(protein phosphorylation);GO:0007169(transmembrane receptor protein tyrosine kinase signaling pathway);GO:0016020(membrane);GO:0016021(integral component of membrane);GO:0016301(kinase activity)04075(Plant hormone signal transduction);04626(Plant-pathogen interaction)K13416EC:2.7.10.1;EC:2.7.11.1expressed protein3.550.5900000000001.150.200.460.78upno
39jchr1+5843163576MSTRG.7.174147MSTRG.7LOC_Os01g01120NANANANANA14.382.4018.272.9411.231.805.800.987.881.234.570.751.670.740.030.56upno
410chr1+6205963576LOC_Os01g01120.141206MSTRG.7LOC_Os01g01120GO:0000287(magnesium ion binding);GO:0005737(cytoplasm);GO:0005829(cytosol);GO:0008270(zinc ion binding);GO:0009507(chloroplast);GO:0009570(chloroplast stroma);GO:0016787(hydrolase activity);GO:0019284(L-methionine biosynthetic process from S-adenosylmethionine);GO:0019509(L-methionine biosynthetic process from methylthioadenosine);GO:0043715(2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity);GO:0043716(2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity);GO:0043874(acireductone synthase activity);GO:0046570(methylthioribulose 1-phosphate dehydratase activity)00270(Cysteine and methionine metabolism)K09880EC:3.1.3.77enolase-phosphatase E1, putative, expressed16.682.7917.542.8225.994.1616.522.788.601.356.601.091.680.740.010.47upno
420chr1-6324466302LOC_Os01g01130.172002MSTRG.8LOC_Os01g01130GO:0005634(nucleus)03013(RNA transport)K13151NAsnurportin-1, putative, expressed31.685.3017.592.8340.196.4330.535.1427.094.2422.843.761.100.130.650.86upno
440chr1+7277577699LOC_Os01g01150.133756MSTRG.10LOC_Os01g01150GO:0000166(nucleotide binding);GO:0003676(nucleic acid binding);GO:0003723(RNA binding);GO:0005634(nucleus);GO:0008150(biological_process);GO:0046872(metal ion binding)NANANARNA recognition motif, putative, expressed18.373.070013.542.179.771.6413.052.0423.463.860.69-0.530.550.81downno
450chr1+7277578702LOC_Os01g01150.244638MSTRG.10LOC_Os01g01150GO:0000166(nucleotide binding);GO:0003676(nucleic acid binding);GO:0003723(RNA binding);GO:0005634(nucleus);GO:0008150(biological_process);GO:0046872(metal ion binding)NANANARNA recognition motif, putative, expressed2.790.471.210.196.331.014.770.804.980.784.530.750.88-0.180.380.78downno
460chr1+7277579938LOC_Os01g01150.354603MSTRG.10LOC_Os01g01150GO:0000166(nucleotide binding);GO:0003676(nucleic acid binding);GO:0003723(RNA binding);GO:0005634(nucleus);GO:0008150(biological_process);GO:0046872(metal ion binding)NANANARNA recognition motif, putative, expressed3.090.527.561.226.140.986.521.104.710.744.970.821.020.020.910.97upno
47jchr1+7277580049MSTRG.10.164241MSTRG.10LOC_Os01g01150NANANANANA12.962.1721.343.4314.082.2516.722.8214.042.207.221.191.210.280.440.78upno
48jchr1+7278679891MSTRG.10.663995MSTRG.10LOC_Os01g01150NANANANANA3.820.6410.381.677.531.214.570.7711.711.8310.361.710.88-0.190.600.84downno
49jchr1+7278679891MSTRG.10.773951MSTRG.10LOC_Os01g01150NANANANANA5.710.9516.772.7013.712.197.511.2711.361.7810.461.721.110.150.690.88upno
50jchr1+7278680049MSTRG.10.564239MSTRG.10LOC_Os01g01150NANANANANA5.230.877.971.288.751.4011.051.8614.902.3310.331.700.74-0.430.070.68downno
51jchr1+7280479846MSTRG.10.854385MSTRG.10LOC_Os01g01150NANANANANA23.873.991.430.237.191.156.681.123.040.48001.650.720.420.78upno
52jchr1+7281780049MSTRG.10.1074001MSTRG.10LOC_Os01g01150NANANANANA2.190.374.900.794.690.757.491.268.431.328.691.430.70-0.520.040.62downno
53jchr1+7281780049MSTRG.10.973990MSTRG.10LOC_Os01g01150NANANANANA3.530.596.971.122.430.3913.702.318.741.376.591.090.66-0.590.150.74downno
540chr1+8242884302LOC_Os01g01160.121090MSTRG.9LOC_Os01g01160GO:0005515(protein binding);GO:0005634(nucleus);GO:0006457(protein folding);GO:0009507(chloroplast);GO:0010322(regulation of isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway);GO:0061077(chaperone-mediated protein folding);GO:1902395(regulation of 1-deoxy-D-xylulose-5-phosphate synthase activity)04141(Protein processing in endoplasmic reticulum)K09518NAheat shock protein DnaJ, putative, expressed34.855.8318.773.022.340.3731.735.3429.184.5727.734.560.53-0.920.210.74downno
550chr1+8533788844LOC_Os01g01170.172334MSTRG.12LOC_Os01g01170NANANANAexpressed protein11.931.9925.354.0815.252.443.590.607.641.203.710.612.071.050.040.63upyes
56jchr1+8534088935MSTRG.12.272419MSTRG.12LOC_Os01g01170NANANANANA8.351.409.641.556.571.054.250.722.860.451.390.231.610.680.040.62upno
600chr1+134291135685LOC_Os01g01280.131226MSTRG.11LOC_Os01g01280GO:0003674(molecular_function);GO:0008150(biological_process);GO:0009507(chloroplast);GO:0009543(chloroplast thylakoid lumen);GO:0009579(thylakoid);GO:0031977(thylakoid lumen)NANANAexpressed protein7.221.215.420.8713.002.089.521.6012.171.9112.452.050.84-0.260.340.78downno
610chr1+139820141588LOC_Os01g01290.121556LOC_Os01g01290LOC_Os01g01290GO:0003677(DNA binding);GO:0003700(transcription factor activity, sequence-specific DNA binding);GO:0005515(protein binding);GO:0005634(nucleus);GO:0005829(cytosol);GO:0006351(transcription, DNA-templated);GO:0006355(regulation of transcription, DNA-templated);GO:0009738(abscisic acid-activated signaling pathway);GO:0010468(regulation of gene expression);GO:0046982(protein heterodimerization activity);GO:0048586(regulation of long-day photoperiodism, flowering);GO:0051247(positive regulation of protein metabolic process);GO:2000905(negative regulation of starch metabolic process)NANANAhistone-like transcription factor and archaeal histone, putative, expressed000000000.670.11000.97-0.050.420.78downno
620chr1+141936144554LOC_Os01g01295.141793MSTRG.15LOC_Os01g01295GO:0009507(chloroplast)NANANAexpressed protein57.659.6448.857.8622.593.6147.467.9918.232.8657.909.531.020.030.970.99upno
63jchr1+142782144556MSTRG.15.221644MSTRG.15LOC_Os01g01295NANANANANA7.181.205.970.962.500.408.711.477.811.227.221.190.78-0.360.160.74downno
640chr1+145577147852LOC_Os01g01302.191155MSTRG.16LOC_Os01g01302GO:0000287(magnesium ion binding);GO:0004765(shikimate kinase activity);GO:0009073(aromatic amino acid family biosynthetic process);GO:0019632(shikimate metabolic process)01230(Biosynthesis of amino acids);00400(Phenylalanine, tyrosine and tryptophan biosynthesis)K00891EC:2.7.1.71shikimate kinase, putative, expressed43.917.3462.4910.0550.678.1154.089.1144.186.9229.414.841.240.310.330.78upno
65jchr1+145602147854MSTRG.16.281228MSTRG.16LOC_Os01g01302NANANANANA13.632.280.270.0417.742.841.320.226.230.9811.301.861.250.320.720.89upno
660chr1+148045150577LOC_Os01g01307.15979MSTRG.17LOC_Os01g01307GO:0003674(molecular_function);GO:0005576(extracellular region);GO:0005774(vacuolar membrane);GO:0005783(endoplasmic reticulum);GO:0005789(endoplasmic reticulum membrane);GO:0005794(Golgi apparatus);GO:0008150(biological_process);GO:0009506(plasmodesma);GO:0016021(integral component of membrane)04141(Protein processing in endoplasmic reticulum)K13250NAtranslocon-associated protein beta domain containing protein, expressed0030.114.84000000001.710.770.470.78upno
68jchr1+148055150610MSTRG.17.651004MSTRG.17LOC_Os01g01307NANANANANA28.364.7474.3511.9613.472.1638.596.50104.8916.4353.908.870.52-0.950.180.74downno
69jchr1+148055150610MSTRG.17.75988MSTRG.17LOC_Os01g01307NANANANANA22.203.712.030.331.450.231.550.267.511.186.511.071.020.030.960.99upno
70jchr1+148055150610MSTRG.17.861353MSTRG.17LOC_Os01g01307NANANANANA32.485.4314.592.3514.052.2512.362.0816.902.6536.656.030.91-0.140.760.91downno
71jchr1+148055150643MSTRG.17.351037MSTRG.17LOC_Os01g01307NANANANANA154.0725.76101.6316.34157.6625.22127.4521.4632.255.0543.837.222.391.250.100.74upno
72jchr1+148055150643MSTRG.17.461037MSTRG.17LOC_Os01g01307NANANANANA95.2315.9299.6316.02164.7426.35153.8825.9147.097.3735.585.861.910.930.090.74upno
73jchr1+148055150643MSTRG.17.551386MSTRG.17LOC_Os01g01307NANANANANA10.111.6911.201.8021.843.4922.533.7925.684.0225.344.170.67-0.580.060.68downno
74jchr1+148068150571MSTRG.17.951429MSTRG.17LOC_Os01g01307NANANANANA29.965.0148.797.8548.357.7336.586.1640.716.3857.139.400.95-0.080.800.93downno
75jchr1+148072150567MSTRG.17.106800MSTRG.17LOC_Os01g01307NANANANANA21.123.53000.490.080.930.162.630.410.820.141.310.390.640.86upno
760chr1+151409156500LOC_Os01g01312.174165MSTRG.14LOC_Os01g01312GO:0003677(DNA binding);GO:0004386(helicase activity);GO:0005524(ATP binding);GO:0005634(nucleus);GO:0006281(DNA repair);GO:0006351(transcription, DNA-templated);GO:0006355(regulation of transcription, DNA-templated);GO:0006974(cellular response to DNA damage stimulus);GO:0010332(response to gamma radiation)03420(Nucleotide excision repair)K10841NAretrotransposon protein, putative, Ty1-copia subclass, expressed22.813.8136.345.8436.305.8130.905.2019.012.9830.104.961.180.240.450.78upno
77xchr1+163052169672MSTRG.21.151539MSTRG.21LOC_Os01g01340NANANANANA19.493.2624.843.9917.762.8414.812.4914.512.277.941.311.460.550.120.74upno
78xchr1+163052169672MSTRG.21.241426MSTRG.21LOC_Os01g01340NANANANANA3.580.60006.120.980011.001.72001.060.090.900.97upno
79xchr1+163114169672MSTRG.21.351480MSTRG.21LOC_Os01g01340NANANANANA1.840.3110.241.656.401.029.401.580013.352.200.96-0.050.940.98downno
800chr1-168470170381LOC_Os01g01340.221731MSTRG.22LOC_Os01g01340GO:0003674(molecular_function);GO:0008150(biological_process);GO:0009507(chloroplast)04144(Endocytosis)K12198NAlight-induced protein 1-like, putative, expressed84.0614.051.050.170.570.093.090.520018.783.091.280.360.810.93upno
810chr1-168470170389LOC_Os01g01340.131645MSTRG.22LOC_Os01g01340GO:0003674(molecular_function);GO:0008150(biological_process);GO:0009507(chloroplast)04144(Endocytosis)K12198NAlight-induced protein 1-like, putative, expressed42.257.0689.5814.41221.0335.36360.5660.72263.5441.27145.2523.910.46-1.110.050.65downno
82jchr1-168581173195MSTRG.22.383105MSTRG.22LOC_Os01g01350NANANANANA40.636.7938.286.1632.975.2718.423.1023.383.668.461.391.980.990.070.69upno
830chr1-170712173191LOC_Os01g01350.171151MSTRG.22LOC_Os01g01350GO:0005515(protein binding);GO:0005634(nucleus);GO:0005737(cytoplasm);GO:0007034(vacuolar transport);GO:0016192(vesicle-mediated transport)04144(Endocytosis)K12198NASNF7 domain containing protein, putative, expressed22.293.736.721.0832.125.149.771.649.481.4815.882.611.410.500.430.78upno
840chr1-170712173434LOC_Os01g01350.251574MSTRG.22LOC_Os01g01350GO:0005515(protein binding);GO:0005634(nucleus);GO:0005737(cytoplasm);GO:0007034(vacuolar transport);GO:0016192(vesicle-mediated transport)04144(Endocytosis)K12198NASNF7 domain containing protein, putative, expressed2.590.435.690.915.130.827.041.194.830.762.630.430.99-0.010.970.99downno
850chr1+178310180812LOC_Os01g01360.112503LOC_Os01g01360LOC_Os01g01360GO:0005215(transporter activity);GO:0005886(plasma membrane);GO:0006857(oligopeptide transport);GO:0016020(membrane);GO:0016021(integral component of membrane)NANANApeptide transporter PTR2, putative, expressed5.870.982.930.474.880.78002.110.331.510.251.450.530.070.69upno
86pchr1-185073185970MSTRG.18.11898MSTRG.18LOC_Os01g01369NANANANANA22.103.6919.093.0720.893.3416.042.7020.413.2015.952.631.130.180.170.74upno
87jchr1-186138190825MSTRG.19.151550MSTRG.19LOC_Os01g01369NANANANANA0033.765.43000000001.760.810.470.78upno
88jchr1-186240188981MSTRG.19.222082MSTRG.19LOC_Os01g01369NANANANANA007.541.2111.071.772.030.348.641.3510.811.780.89-0.160.820.93downno
890chr1-186246190846LOC_Os01g01369.141545MSTRG.19LOC_Os01g01369GO:0000247(C-8 sterol isomerase activity);GO:0005789(endoplasmic reticulum membrane);GO:0005886(plasma membrane);GO:0016021(integral component of membrane);GO:0016126(sterol biosynthetic process);GO:0047750(cholestenol delta-isomerase activity)00100(Steroid biosynthesis)K01824EC:5.3.3.53-beta-hydroxysteroid-Delta-isomerase, putative, expressed91.0315.2236.375.8542.986.8773.8112.4393.1714.5974.0912.200.66-0.590.260.78downno
900chr1+191036196287LOC_Os01g01380.163482MSTRG.20LOC_Os01g01380NANANANAexpressed protein2.600.444.120.665.400.863.890.654.220.664.750.780.98-0.030.800.93downno
910chr1+197647200803LOC_Os01g01390.142142MSTRG.23LOC_Os01g01390GO:0003674(molecular_function);GO:0005634(nucleus);GO:0005886(plasma membrane);GO:0008150(biological_process)NANANAexpressed protein108.4218.1397.4415.67113.9418.2389.8915.14104.3716.3450.398.291.360.450.240.77upno
920chr1+197647200803LOC_Os01g01390.252175MSTRG.23LOC_Os01g01390GO:0003674(molecular_function);GO:0005634(nucleus);GO:0005886(plasma membrane);GO:0008150(biological_process)NANANAexpressed protein00009.651.540.060.010.090.01001.440.530.210.74upno
930chr1+197647200803LOC_Os01g01390.352182MSTRG.23LOC_Os01g01390GO:0003674(molecular_function);GO:0005634(nucleus);GO:0005886(plasma membrane);GO:0008150(biological_process)NANANAexpressed protein42.827.1615.402.4830.644.9011.581.950.300.0524.894.102.211.140.290.78upno
940chr1+197647200803LOC_Os01g01390.442261MSTRG.23LOC_Os01g01390GO:0003674(molecular_function);GO:0005634(nucleus);GO:0005886(plasma membrane);GO:0008150(biological_process)NANANAexpressed protein008.731.4017.492.80009.011.4112.362.031.080.120.900.97upno
950chr1+197647200803LOC_Os01g01390.531054MSTRG.23LOC_Os01g01390GO:0003674(molecular_function);GO:0005634(nucleus);GO:0005886(plasma membrane);GO:0008150(biological_process)NANANAexpressed protein7.341.238.101.306.461.031.900.322.860.457.621.251.310.380.180.74upno
960chr1+201944206743LOC_Os01g01400.1122735MSTRG.26LOC_Os01g01400GO:0003674(molecular_function);GO:0005575(cellular_component);GO:0008150(biological_process)NANANAexpressed protein3.960.663.520.579.871.585.800.982.410.382.180.361.280.360.010.43upno
970chr1-205710209606LOC_Os01g01410.142489MSTRG.27LOC_Os01g01410GO:0004674(protein serine/threonine kinase activity);GO:0005886(plasma membrane)04626(Plant-pathogen interaction)K13430EC:2.7.11.1TKL_IRAK_C-LEC.1 - TKL_IRAK_C-LEC has homology to C-type lectin receptors, expressed73.2312.2457.949.3277.4412.3923.483.9576.5911.9924.123.971.770.820.230.76upno
980chr1-205710209606LOC_Os01g01410.242486MSTRG.27LOC_Os01g01410GO:0004674(protein serine/threonine kinase activity);GO:0005886(plasma membrane)04626(Plant-pathogen interaction)K13430EC:2.7.11.1TKL_IRAK_C-LEC.1 - TKL_IRAK_C-LEC has homology to C-type lectin receptors, expressed38.376.4243.937.0665.8210.5320.303.4232.015.0116.472.711.970.980.040.62upno
99jchr1-205711209582MSTRG.27.442528MSTRG.27LOC_Os01g01410NANANANANA001.090.18000000001.050.070.470.78upno
100jchr1-205711209600MSTRG.27.332613MSTRG.27LOC_Os01g01410NANANANANA20.573.4416.782.705.000.8012.102.047.541.187.391.221.220.280.560.82upno
101jchr1+209677214224MSTRG.28.172891MSTRG.28LOC_Os01g01420NANANANANA3.110.526.931.1125.934.1543.787.3710.711.6826.694.390.58-0.800.170.74downno
1020chr1+209771214188LOC_Os01g01420.181657MSTRG.28LOC_Os01g01420GO:0005720(nuclear heterochromatin);GO:0010216(maintenance of DNA methylation);GO:0016567(protein ubiquitination);GO:0061630(ubiquitin protein ligase activity);GO:0090308(regulation of methylation-dependent chromatin silencing)NANANAexpressed protein93.4415.62120.5419.38124.9219.9811.441.9364.7110.1390.2614.862.221.150.210.74upno
1030chr1+209771214188LOC_Os01g01420.381653MSTRG.28LOC_Os01g01420GO:0005720(nuclear heterochromatin);GO:0010216(maintenance of DNA methylation);GO:0016567(protein ubiquitination);GO:0061630(ubiquitin protein ligase activity);GO:0090308(regulation of methylation-dependent chromatin silencing)NANANAexpressed protein14.212.3812.211.9635.675.7113.572.2822.603.5443.157.100.83-0.270.690.88downno
1040chr1+209903214229LOC_Os01g01420.271913MSTRG.28LOC_Os01g01420GO:0005720(nuclear heterochromatin);GO:0010216(maintenance of DNA methylation);GO:0016567(protein ubiquitination);GO:0061630(ubiquitin protein ligase activity);GO:0090308(regulation of methylation-dependent chromatin silencing)NANANAexpressed protein14.532.438.241.3340.576.49115.1419.3928.404.4530.234.980.51-0.960.070.68downno
1050chr1+216126217664LOC_Os01g01430.121425MSTRG.24LOC_Os01g01430GO:0003700(transcription factor activity, sequence-specific DNA binding);GO:0005634(nucleus);GO:0006351(transcription, DNA-templated);GO:0006355(regulation of transcription, DNA-templated);GO:0007275(multicellular organism development);GO:0009753(response to jasmonic acid);GO:0044212(transcription regulatory region DNA binding);GO:0045995(regulation of embryonic development);GO:0048317(seed morphogenesis);GO:0080060(integument development)00561(Glycerolipid metabolism)K01054EC:3.1.1.23No apical meristem protein, putative, expressed0.210.0300007.361.24000.830.140.77-0.380.350.78downno

document location:

summary/5_differential_expression/*VS*/Gene_differential_expression.xlsx

summary/5_differential_expression/*VS*/Transcript_differential_expression.xlsx


4.7.2 Volcano of differentially expressed genes/transcripts




document location:

summary/5_differential_expression/*VS*/*_Genes_volcano.png

summary/5_differential_expression/*VS*/*_Transcript_volcano.png


4.7.3 Heatmap of differentially expressed genes/transcripts


Heatmap is a graphical representation of data where the individual z values (repeated) or Log10 (non-repeated) contained in a matrix are represented as color scheme. Calculation formula of z value is shown below. Sample-i means expression level (fpkm value).





document location:

summary/5_differential_expression/*VS*/*_genes_heatmap.png

summary/5_differential_expression/*VS*/*_transcripts_heatmap.png


4.7.4 GO enrichment analysis of differentially expressed genes


Gene ontology (GO) (http://www.geneontology.org) is a major bioinformatics initiative to unify the representation of gene and gene product attributes across all species. More specifically, the project aims to: 1) maintain and develop its controlled vocabulary of gene and gene product attributes; 2) annotate genes and gene products, and assimilate and disseminate annotation data; and 3) provide tools for easy access to all aspects of the data provided by the project, and to enable functional interpretation of experimental data using the GO, for example via enrichment analysis.

GO is part of a larger classification effort, the Open Biomedical Ontologies (OBO).

Although gene nomenclature itself aims to maintain and develop controlled vocabulary of gene and gene products, the Gene Ontology extends the effort by using markup language to make the data (not only of the genes and their products but also of all their attributes) machine readable, and to do so in a way that is unified across all species (whereas gene nomenclature conventions vary by biologic taxon).

Significant GO terms were calculated by Hypergeometric equation as shown below. TB gene number=number of total genes; TS gene number=number of differentially expressed genes in total genes; B gene number=total number of gene in all GO terms; S gene number=number of differentially expressed genes in this GO term. Those GO terms with p value<0.05 were defined as significant GO terms.



Significant GO terms::

GO_IDGO_TermGO_functionGO_classGenesS gene numberTS gene numberB gene numberTB gene numberpvalue
GO:0003677DNA bindingmolecular_function6LOC_Os01g11550;LOC_Os01g14720;LOC_Os01g36390;LOC_Os01g43230;LOC_Os01g44390;LOC_Os01g45090;LOC_Os01g64360;LOC_Os02g34970;LOC_Os02g49986;LOC_Os02g58440;LOC_Os03g03260;LOC_Os03g09170;LOC_Os03g20090;LOC_Os03g20900;LOC_Os03g53190;LOC_Os03g57260;LOC_Os03g60080;LOC_Os04g32590;LOC_Os04g34970;LOC_Os04g45330;LOC_Os05g02420;LOC_Os05g23700;LOC_Os05g32070;LOC_Os06g03670;LOC_Os06g22030;LOC_Os06g46560;LOC_Os08g33150;LOC_Os09g04440;LOC_Os09g28200;LOC_Os10g39150;LOC_Os11g03420;LOC_Os12g06200;LOC_Os12g29330;LOC_Os12g34510;LOC_Os12g3740035298977178830.00
GO:0006268DNA unwinding involved in DNA replicationbiological_process11LOC_Os01g36390;LOC_Os11g29380;LOC_Os12g3740032987178830.00
GO:0015979photosynthesisbiological_process5LOC_Os01g14410;LOC_Os01g41710;LOC_Os03g31750;LOC_Os04g59440;LOC_Os07g05365;LOC_Os07g05480629855178830.00
GO:0016168chlorophyll bindingmolecular_function6LOC_Os01g14410;LOC_Os01g41710;LOC_Os04g59440;LOC_Os07g05480429823178830.00
GO:0009534chloroplast thylakoidcellular_component12LOC_Os01g02880;LOC_Os01g31690;LOC_Os03g49720;LOC_Os04g59440;LOC_Os07g05365;LOC_Os07g05480;LOC_Os10g39150729889178830.00
GO:0010025wax biosynthetic processbiological_process5LOC_Os02g56920;LOC_Os03g14170;LOC_Os10g33250329812178830.00
GO:0015140malate transmembrane transporter activitymolecular_function10LOC_Os02g45150;LOC_Os04g34010;LOC_Os10g42180329812178830.00
GO:0043068positive regulation of programmed cell deathbiological_process8LOC_Os02g07430;LOC_Os05g41670;LOC_Os06g46560329813178830.00
GO:0008094DNA-dependent ATPase activitymolecular_function11LOC_Os01g36390;LOC_Os12g31370;LOC_Os12g37400329814178830.00
GO:0009523photosystem IIcellular_component10LOC_Os01g14410;LOC_Os04g59440;LOC_Os07g05365329814178830.00
GO:0008453alanine-glyoxylate transaminase activitymolecular_function7LOC_Os03g21960;LOC_Os08g3930022984178830.00
GO:0042555MCM complexcellular_component5LOC_Os01g36390;LOC_Os12g3740022984178830.00
GO:0007049cell cyclebiological_process5LOC_Os01g36390;LOC_Os09g02360;LOC_Os09g21450;LOC_Os12g06200;LOC_Os12g37400529857178830.00
GO:0043668exinecellular_component6LOC_Os02g56920;LOC_Os10g3325022985178830.00
GO:0009737response to abscisic acidbiological_process6LOC_Os03g01320;LOC_Os03g20090;LOC_Os03g58790;LOC_Os04g34970;LOC_Os04g39110;LOC_Os04g43200;LOC_Os08g33150;LOC_Os09g25390;LOC_Os10g39150;LOC_Os11g06980;LOC_Os12g2933011298251178830.00
GO:0010287plastoglobulecellular_component13LOC_Os01g02880;LOC_Os01g31690;LOC_Os01g41710;LOC_Os03g49720429838178830.00
GO:0009651response to salt stressbiological_process6LOC_Os02g02870;LOC_Os03g01320;LOC_Os03g09170;LOC_Os03g20090;LOC_Os04g39110;LOC_Os04g40600;LOC_Os04g43200;LOC_Os06g08080;LOC_Os07g01020;LOC_Os11g06980;LOC_Os12g2933011298256178830.00
GO:0000347THO complexcellular_component11LOC_Os01g36390;LOC_Os12g3740022987178830.01
GO:0010207photosystem II assemblybiological_process7LOC_Os01g31690;LOC_Os06g2454022988178830.01
GO:0042127regulation of cell proliferationbiological_process5LOC_Os04g43300;LOC_Os09g0236022988178830.01
GO:0009522photosystem Icellular_component10LOC_Os01g14410;LOC_Os07g0548022988178830.01
GO:0003727single-stranded RNA bindingmolecular_function7LOC_Os02g02510;LOC_Os03g1956022988178830.01
GO:0009535chloroplast thylakoid membranecellular_component13LOC_Os01g14410;LOC_Os01g31690;LOC_Os01g41710;LOC_Os03g49720;LOC_Os04g59440;LOC_Os07g05365;LOC_Os07g05480;LOC_Os07g30670;LOC_Os10g391509298204178830.01
GO:0048653anther developmentbiological_process7LOC_Os02g30850;LOC_Os02g56920;LOC_Os10g33250329826178830.01
GO:0031072heat shock protein bindingmolecular_function5LOC_Os02g30320;LOC_Os05g0153022989178830.01
GO:0009705plant-type vacuole membranecellular_component11LOC_Os02g45150;LOC_Os04g34010;LOC_Os06g08080;LOC_Os10g39220;LOC_Os10g42180529878178830.01
GO:0006270DNA replication initiationbiological_process11LOC_Os01g36390;LOC_Os12g37400229810178830.01
GO:0009269response to desiccationbiological_process6LOC_Os04g43200;LOC_Os11g06980229811178830.01
GO:0006351transcription, DNA-templatedbiological_process10LOC_Os01g11550;LOC_Os01g43230;LOC_Os01g44390;LOC_Os01g45090;LOC_Os01g64360;LOC_Os02g34970;LOC_Os03g03260;LOC_Os03g09170;LOC_Os03g20090;LOC_Os03g20900;LOC_Os03g60080;LOC_Os04g32590;LOC_Os04g34970;LOC_Os05g02420;LOC_Os05g48650;LOC_Os05g49100;LOC_Os06g03670;LOC_Os06g22030;LOC_Os09g28200;LOC_Os11g03420;LOC_Os12g06200;LOC_Os12g29330;LOC_Os12g3740023298850178830.02
GO:0009773photosynthetic electron transport in photosystem Ibiological_process8LOC_Os07g30670;LOC_Os10g39150229812178830.02
GO:0003700transcription factor activity, sequence-specific DNA bindingmolecular_function4LOC_Os01g02100;LOC_Os01g11550;LOC_Os01g44390;LOC_Os01g45090;LOC_Os01g64360;LOC_Os01g66290;LOC_Os02g34970;LOC_Os02g49986;LOC_Os03g03260;LOC_Os03g09170;LOC_Os03g20090;LOC_Os03g20900;LOC_Os03g41390;LOC_Os04g32590;LOC_Os04g34970;LOC_Os04g45330;LOC_Os05g02420;LOC_Os05g32070;LOC_Os05g49100;LOC_Os06g03670;LOC_Os06g46560;LOC_Os08g33150;LOC_Os09g28200;LOC_Os12g06200;LOC_Os12g2933025298951178830.02
GO:0009607response to biotic stimulusbiological_process4LOC_Os04g3915012981178830.02
GO:0050281serine-glyoxylate transaminase activitymolecular_function7LOC_Os08g3930012981178830.02
GO:0004558alpha-1,4-glucosidase activitymolecular_function9LOC_Os01g1631012981178830.02
GO:0035304regulation of protein dephosphorylationbiological_process9LOC_Os01g3169012981178830.02
GO:0032450maltose alpha-glucosidase activitymolecular_function9LOC_Os01g1631012981178830.02
GO:0046498S-adenosylhomocysteine metabolic processbiological_process11LOC_Os03g5533012981178830.02
GO:0046906tetrapyrrole bindingmolecular_function5LOC_Os11g1655012981178830.02
GO:0046521sphingoid catabolic processbiological_process8LOC_Os10g2670012981178830.02
GO:0006271DNA strand elongation involved in DNA replicationbiological_process11LOC_Os01g1069012981178830.02
GO:0046922peptide-O-fucosyltransferase activitymolecular_function8LOC_Os07g3849012981178830.02
GO:1901918negative regulation of exoribonuclease activitybiological_process12LOC_Os03g1956012981178830.02
GO:0045860positive regulation of protein kinase activitybiological_process11LOC_Os08g0234012981178830.02
GO:1900033negative regulation of trichome patterningbiological_process10LOC_Os01g4323012981178830.02
GO:0010466negative regulation of peptidase activitybiological_process10LOC_Os04g2222012981178830.02
GO:00086187-methylguanosine metabolic processbiological_process12LOC_Os03g5353012981178830.02
GO:0005658alpha DNA polymerase:primase complexcellular_component13LOC_Os01g6482012981178830.02
GO:0042493response to drugbiological_process5LOC_Os08g3955012981178830.02
GO:0050306sucrose 1F-fructosyltransferase activitymolecular_function8LOC_Os02g0159012981178830.02
GO:0010242oxygen evolving activitymolecular_function5LOC_Os01g3169012981178830.02
GO:0009173pyrimidine ribonucleoside monophosphate metabolic processbiological_process11LOC_Os06g0200012981178830.02
GO:0030054cell junctioncellular_component3LOC_Os05g3001012981178830.02
GO:0051446positive regulation of meiotic cell cyclebiological_process6LOC_Os12g0620012981178830.02
GO:0004760serine-pyruvate transaminase activitymolecular_function7LOC_Os08g3930012981178830.02
GO:0048629trichome patterningbiological_process8LOC_Os01g4323012981178830.02
GO:0042549photosystem II stabilizationbiological_process8LOC_Os01g3169012981178830.02
GO:0033609oxalate metabolic processbiological_process9LOC_Os08g08970;LOC_Os08g08980;LOC_Os08g13440329833178830.02
GO:0046564oxalate decarboxylase activitymolecular_function7LOC_Os08g08970;LOC_Os08g08980;LOC_Os08g13440329833178830.02
GO:2000652regulation of secondary cell wall biogenesisbiological_process8LOC_Os02g49986;LOC_Os08g33150229813178830.02
GO:0000790nuclear chromatincellular_component13LOC_Os03g53190;LOC_Os12g34510229813178830.02
GO:0009579thylakoidcellular_component7LOC_Os01g31690;LOC_Os04g59440;LOC_Os07g05365;LOC_Os07g05480;LOC_Os10g39150529893178830.02
GO:0022625cytosolic large ribosomal subunitcellular_component11LOC_Os01g51020;LOC_Os02g32760;LOC_Os02g47140;LOC_Os07g33997;LOC_Os08g02340;LOC_Os09g325206298129178830.02
GO:0009654photosystem II oxygen evolving complexcellular_component10LOC_Os01g31690;LOC_Os07g05365229814178830.02
GO:0003697single-stranded DNA bindingmolecular_function8LOC_Os02g02510;LOC_Os02g02870;LOC_Os12g31370329837178830.02
GO:0006355regulation of transcription, DNA-templatedbiological_process11LOC_Os01g11550;LOC_Os01g14720;LOC_Os01g44390;LOC_Os01g45090;LOC_Os01g64360;LOC_Os02g02870;LOC_Os02g34970;LOC_Os02g49986;LOC_Os03g03260;LOC_Os03g09170;LOC_Os03g20090;LOC_Os03g20900;LOC_Os03g60080;LOC_Os04g32590;LOC_Os04g34970;LOC_Os05g02420;LOC_Os05g23700;LOC_Os05g49100;LOC_Os06g03670;LOC_Os06g05890;LOC_Os06g22030;LOC_Os06g39680;LOC_Os06g46560;LOC_Os08g33150;LOC_Os09g28200;LOC_Os11g03420;LOC_Os12g06200;LOC_Os12g37400282981132178830.02
GO:0010427abscisic acid bindingmolecular_function6LOC_Os02g15640;LOC_Os08g28670229815178830.03
GO:0080163regulation of protein serine/threonine phosphatase activitybiological_process11LOC_Os02g15640;LOC_Os08g28670229815178830.03
GO:0070180large ribosomal subunit rRNA bindingmolecular_function8LOC_Os02g32760;LOC_Os08g02340229815178830.03
GO:0048481plant ovule developmentbiological_process8LOC_Os01g66290;LOC_Os03g03260229816178830.03
GO:0004864protein phosphatase inhibitor activitymolecular_function6LOC_Os02g15640;LOC_Os08g28670229816178830.03
GO:0009620response to fungusbiological_process7LOC_Os03g20090;LOC_Os03g60080;LOC_Os04g43200329841178830.03
GO:0005509calcium ion bindingmolecular_function7LOC_Os01g31690;LOC_Os02g55880;LOC_Os04g43200;LOC_Os05g31620;LOC_Os09g25390;LOC_Os09g310406298141178830.03
GO:0048283indeterminate inflorescence morphogenesisbiological_process6LOC_Os01g6629012982178830.03
GO:0003917DNA topoisomerase type I activitymolecular_function6LOC_Os03g5726012982178830.03
GO:0006272leading strand elongationbiological_process12LOC_Os01g6482012982178830.03
GO:0031407oxylipin metabolic processbiological_process10LOC_Os04g4320012982178830.03
GO:0031990mRNA export from nucleus in response to heat stressbiological_process8LOC_Os02g0251012982178830.03
GO:0007051spindle organizationbiological_process7LOC_Os05g2829012982178830.03
GO:0050242pyruvate, phosphate dikinase activitymolecular_function7LOC_Os03g3175012982178830.03
GO:0046500S-adenosylmethionine metabolic processbiological_process11LOC_Os03g5533012982178830.03
GO:0006090pyruvate metabolic processbiological_process9LOC_Os03g3175012982178830.03
GO:0051782negative regulation of cell divisionbiological_process7LOC_Os08g4260012982178830.03
GO:0030295protein kinase activator activitymolecular_function6LOC_Os08g0234012982178830.03
GO:0051091positive regulation of sequence-specific DNA binding transcription factor activitybiological_process13LOC_Os01g5941012982178830.03
GO:0070131positive regulation of mitochondrial translationbiological_process10LOC_Os05g0133012982178830.03
GO:0015099nickel cation transmembrane transporter activitymolecular_function11LOC_Os03g0609012982178830.03
GO:0071614linoleic acid epoxygenase activitymolecular_function6LOC_Os04g4320012982178830.03
GO:0004127cytidylate kinase activitymolecular_function9LOC_Os06g0200012982178830.03
GO:0032578aleurone grain membranecellular_component12LOC_Os03g2682012982178830.03
GO:0009517PSII associated light-harvesting complex IIcellular_component13LOC_Os04g5944012982178830.03
GO:0046604positive regulation of mitotic centrosome separationbiological_process12LOC_Os05g2829012982178830.03
GO:0034402recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complexbiological_process12LOC_Os02g0251012982178830.03
GO:0009041uridylate kinase activitymolecular_function9LOC_Os06g0200012982178830.03
GO:0015675nickel cation transportbiological_process9LOC_Os03g0609012982178830.03
GO:0051738xanthophyll bindingmolecular_function6LOC_Os04g5944012982178830.03
GO:0000082G1/S transition of mitotic cell cyclebiological_process8LOC_Os05g2829012982178830.03
GO:0010114response to red lightbiological_process8LOC_Os01g44390;LOC_Os03g19560229818178830.04
GO:0009409response to coldbiological_process5LOC_Os01g14410;LOC_Os02g02870;LOC_Os03g01320;LOC_Os03g09170;LOC_Os03g14170;LOC_Os04g43200;LOC_Os06g036707298185178830.04
GO:0031969chloroplast membranecellular_component13LOC_Os03g19560;LOC_Os04g43200;LOC_Os11g16550329844178830.04

document location: summary/5_differential_expression/*VS*/1_GO_Enrichment/*VS*_GO_enrichment_Gene.xlsx


Barplot of enriched GO terms:



document location: summary/5_differential_expression/*VS*/1_GO_Enrichment/*VS*_GO_enrichment.png


Scatterplot of enriched GO terms:

Number of differentially expressed genes enriched in GO terms, p value and rich factor are shown in scatterplot. Rich factor=(number of differentially expressed genes in GO term)/(total number of genes in GO term). The larger rich factor is, the higher enrichment is.




document location: summary/5_differential_expression/*VS*/1_GO_Enrichment/*VS*_GO_enrichment_scatterplot.png


4.7.5 KEGG enrichment analysis of differentially expressed genes


KEGG (Kyoto Encyclopedia of Genes and Genomes) (http://www.kegg.jp/) is a collection of databases dealing with genomes, biological pathways, diseases, drugs, and chemical substances. KEGG is utilized for bioinformatics research and education, including data analysis in genomics, metagenomics, metabolomics and other omics studies, modeling and simulation in systems biology, and translational research in drug development.

Significant KEGG pathways were calculated by Hypergeometric equation as shown below. TB gene number=number of total genes; TS gene number=number of differentially expressed genes in total genes; B gene number=total number of genes in KEGG pathways; S gene number=number of differentially expressed genes in this KEGG pathway. Those KEGG pathways with p value<0.05 were defined as significant KEGG pathways.



Significant KEGG pathways::

pathway_idpathway_nameGenesS gene numberTS gene numberB gene numberTB gene numberpvalue
ko03030DNA replicationLOC_Os01g10690;LOC_Os01g36390;LOC_Os01g64820;LOC_Os02g58220;LOC_Os03g57260;LOC_Os03g63870;LOC_Os11g29380;LOC_Os12g37400816210198200.00
ko03010RibosomeLOC_Os01g08470;LOC_Os01g51020;LOC_Os01g73160;LOC_Os02g01540;LOC_Os02g32760;LOC_Os02g47140;LOC_Os03g01320;LOC_Os03g07290;LOC_Os03g59720;LOC_Os05g39960;LOC_Os07g33997;LOC_Os08g02340;LOC_Os09g325201316238798200.01
ko03060Protein exportLOC_Os02g54060;LOC_Os05g43540;LOC_Os10g37690;LOC_Os11g0130041626798200.02
ko00603Glycosphingolipid biosynthesis - globo seriesLOC_Os10g35070;LOC_Os12g0555021621798200.03
ko00195PhotosynthesisLOC_Os01g31690;LOC_Os04g59440;LOC_Os07g05365;LOC_Os07g0548041627498200.03
ko00073Cutin, suberine and wax biosynthesisLOC_Os01g42870;LOC_Os02g56920;LOC_Os04g43200;LOC_Os10g3325041627598200.04
ko03440Homologous recombinationLOC_Os01g10690;LOC_Os02g58220;LOC_Os03g57260;LOC_Os03g63870;LOC_Os12g31370516212198200.05
ko03430Mismatch repairLOC_Os01g10690;LOC_Os02g58220;LOC_Os03g57260;LOC_Os03g6387041628498200.05
ko00280Valine, leucine and isoleucine degradationLOC_Os03g10620;LOC_Os03g21960;LOC_Os06g5038031625298200.05
ko00040Pentose and glucuronate interconversionsLOC_Os01g44390;LOC_Os05g37060;LOC_Os06g01760;LOC_Os06g50380;LOC_Os08g12410;LOC_Os11g08750616216598200.06
ko00943Isoflavonoid biosynthesisLOC_Os08g37040;LOC_Os09g2869021622798200.07
ko00750Vitamin B6 metabolismLOC_Os02g13290;LOC_Os07g0102021623898200.13
ko00966Glucosinolate biosynthesisLOC_Os04g08828;LOC_Os04g0943021624098200.14
ko00904Diterpenoid biosynthesisLOC_Os05g48700;LOC_Os07g46852;LOC_Os09g0745031628098200.15
ko00860Porphyrin and chlorophyll metabolismLOC_Os01g10930;LOC_Os02g49986;LOC_Os03g58790;LOC_Os08g33150416212398200.15
ko00250Alanine, aspartate and glutamate metabolismLOC_Os03g21960;LOC_Os08g3930021624298200.15
ko00460Cyanoamino acid metabolismLOC_Os04g08828;LOC_Os04g09430;LOC_Os04g32540;LOC_Os11g31980416212798200.16
ko00196Photosynthesis - antenna proteinsLOC_Os01g4171011621198200.17
ko00240Pyrimidine metabolismLOC_Os01g10690;LOC_Os01g64820;LOC_Os02g02510;LOC_Os03g56310;LOC_Os06g02000516218098200.18
ko00510N-Glycan biosynthesisLOC_Os01g16310;LOC_Os01g5784021624998200.19
ko03013RNA transportLOC_Os01g03490;LOC_Os01g66290;LOC_Os02g02870;LOC_Os03g14030;LOC_Os03g20900;LOC_Os04g42140;LOC_Os05g28510;LOC_Os09g04440816234598200.21
ko00561Glycerolipid metabolismLOC_Os02g34970;LOC_Os03g60080;LOC_Os06g50380;LOC_Os10g35070416215098200.24
ko00500Starch and sucrose metabolismLOC_Os01g44390;LOC_Os01g71474;LOC_Os02g01590;LOC_Os03g14540;LOC_Os03g28330;LOC_Os03g30000;LOC_Os05g37060;LOC_Os06g01760;LOC_Os07g35480;LOC_Os08g02996;LOC_Os08g12410;LOC_Os11g087501216258698200.26
ko03420Nucleotide excision repairLOC_Os01g10690;LOC_Os02g58220;LOC_Os03g57260;LOC_Os03g63870416216998200.30
ko00340Histidine metabolismLOC_Os06g5038011622398200.32
ko00941Flavonoid biosynthesisLOC_Os07g40986;LOC_Os08g43040;LOC_Os10g40934316212298200.33
ko00640Propanoate metabolismLOC_Os03g1062011622698200.35
ko00760Nicotinate and nicotinamide metabolismLOC_Os03g5631011622698200.35
ko04933AGE-RAGE signaling pathway in diabetic complicationsLOC_Os02g02840;LOC_Os06g0589021627598200.35
ko03015mRNA surveillance pathwayLOC_Os01g66290;LOC_Os08g38880;LOC_Os09g04440;LOC_Os09g37790416218398200.36
ko00230Purine metabolismLOC_Os01g10690;LOC_Os01g64820;LOC_Os02g02510;LOC_Os03g56310416218898200.38
ko00590Arachidonic acid metabolismLOC_Os01g7043011623098200.39
ko04145PhagosomeLOC_Os02g02840;LOC_Os05g01810;LOC_Os11g01300316213898200.40
ko04141Protein processing in endoplasmic reticulumLOC_Os01g16310;LOC_Os01g57840;LOC_Os02g54060;LOC_Os03g63520;LOC_Os04g46390;LOC_Os05g01520;LOC_Os11g01300716236698200.40
ko00902MonoterpenoidLOC_Os03g5288011623298200.41
ko00052Galactose metabolismLOC_Os02g01590;LOC_Os10g3507021628798200.42
ko00410beta-Alanine metabolismLOC_Os03g10620;LOC_Os06g5038021628898200.43
ko04075Plant hormone signal transductionLOC_Os02g15640;LOC_Os02g56120;LOC_Os04g22220;LOC_Os04g52000;LOC_Os04g55620;LOC_Os05g51660;LOC_Os06g22919;LOC_Os06g50380;LOC_Os08g41320;LOC_Os09g02360;LOC_Os09g21450;LOC_Os09g375001216268298200.45
ko00010Glycolysis / GluconeogenesisLOC_Os01g02880;LOC_Os05g39310;LOC_Os06g50380316215198200.46
ko00604Glycosphingolipid biosynthesis - ganglio seriesLOC_Os12g0555011623798200.46
ko012102-Oxocarboxylic acid metabolismLOC_Os04g08828;LOC_Os04g0943021629498200.46
ko00620Pyruvate metabolismLOC_Os03g31750;LOC_Os06g5038021629498200.46
ko00071Fatty acid degradationLOC_Os06g5038011623898200.47
ko00514Other types of O-glycanLOC_Os07g3849011623998200.48
ko04931Insulin resistanceLOC_Os04g52000;LOC_Os06g50380;LOC_Os12g01140316215898200.49
ko00910Nitrogen metabolismLOC_Os08g3663011624298200.50
ko00480Glutathione metabolismLOC_Os01g27360;LOC_Os03g04220216210298200.50
ko04070Phosphatidylinositol signaling systemLOC_Os03g53190;LOC_Os12g34510216210298200.50
ko04626Plant-pathogen interactionLOC_Os01g45090;LOC_Os01g53920;LOC_Os03g09170;LOC_Os03g20090;LOC_Os03g20450;LOC_Os03g53190;LOC_Os04g22220;LOC_Os04g34970;LOC_Os05g02420;LOC_Os05g07300;LOC_Os05g31620;LOC_Os05g49100;LOC_Os05g51660;LOC_Os06g46560;LOC_Os07g08750;LOC_Os08g34640;LOC_Os08g39550;LOC_Os09g39930;LOC_Os10g33080;LOC_Os12g3451020162119498200.51
ko00920Sulfur metabolismLOC_Os02g5588011624398200.51
ko00310Lysine degradationLOC_Os06g5038011624598200.53
ko04146PeroxisomeLOC_Os08g39300;LOC_Os12g32330216210898200.53
ko00100Steroid biosynthesisLOC_Os01g5352011624798200.54
ko00563Glycosylphosphatidylinositol(GPI)-anchor biosynthesisLOC_Os01g5511011624898200.55
ko00270Cysteine and methionine metabolismLOC_Os03g21960;LOC_Os10g28350216211598200.57
ko00710Carbon fixation in photosynthetic organismsLOC_Os01g02880;LOC_Os03g31750216211898200.58
ko00260Glycine, serine and threonine metabolismLOC_Os03g21960;LOC_Os08g39300216211898200.58
ko00565Ether lipid metabolismLOC_Os09g2539011625398200.59
ko00903Limonene and pineneLOC_Os06g5038011625698200.61
ko00531Glycosaminoglycan degradationLOC_Os02g4463011625798200.61
ko00330Arginine and proline metabolismLOC_Os06g5038011626098200.63
ko03040SpliceosomeLOC_Os02g04950;LOC_Os03g19560;LOC_Os03g49200;LOC_Os06g50890;LOC_Os07g04150;LOC_Os07g10256616240098200.65
ko03050ProteasomeLOC_Os08g4364011627398200.70
ko03020RNA polymeraseLOC_Os02g0251011627398200.70
ko00630Glyoxylate and dicarboxylate metabolismLOC_Os08g3930011627698200.72
ko00600Sphingolipid metabolismLOC_Os10g3507011627798200.72
ko00030Pentose phosphate pathwayLOC_Os01g0288011627998200.73
ko01200Carbon metabolismLOC_Os01g02880;LOC_Os03g10620;LOC_Os03g31750;LOC_Os08g39300416231498200.77
ko04140Regulation of autophagyLOC_Os12g4118011629098200.78
ko03410Base excision repairLOC_Os01g1069011629198200.78
ko00190Oxidative phosphorylationLOC_Os02g49986;LOC_Os06g08080;LOC_Os08g33150316224998200.78
ko00053Ascorbate and aldarate metabolismLOC_Os06g5038011629498200.79
ko03018RNA degradationLOC_Os03g40020;LOC_Os04g40600;LOC_Os12g29330316225398200.79
ko00592alpha-Linolenic acid metabolismLOC_Os06g1356011629598200.80
ko00051Fructose and mannose metabolismLOC_Os01g0288011629998200.81
ko00380Tryptophan metabolismLOC_Os06g50380116210598200.83
ko00945Stilbenoid, diarylheptanoid and gingerol biosynthesisLOC_Os08g43040116210798200.83
ko00940Phenylpropanoid biosynthesisLOC_Os03g25300;LOC_Os04g09654;LOC_Os06g46799;LOC_Os08g43040416235898200.85
ko04712Circadian rhythm - plantLOC_Os01g43230116213798200.90
ko04144EndocytosisLOC_Os01g68750;LOC_Os02g29140;LOC_Os03g02750;LOC_Os09g25390416241698200.92
ko00520Amino sugar and nucleotide sugar metabolismLOC_Os01g04580;LOC_Os03g14540;LOC_Os03g30000316234698200.93
ko00564Glycerophospholipid metabolismLOC_Os09g25390116215898200.93
ko00062Fatty acid elongationLOC_Os03g14170116217898200.95
ko01230Biosynthesis of amino acidsLOC_Os01g02880116230698200.99

document location: summary/5_differential_expression/*VS*/2_KEGG_Enrichment/*VS*_KEGG_enrichment_Gene.xlsx


Scatterplot of enriched KEGG pathways:

Number of differentially expressed genes enriched in KEGG pathway, p value and rich factor are shown in scatterplot. Rich factor=(number of differentially expressed genes in KEGG pathway)/(total number of genes in KEGG pathway). The larger rich factor is, the higher enrichment is.




document location:

summary/5_differential_expression/*VS*/2_KEGG_Enrichment/*VS*_KEGG_enrichment_scatterplot.png



KEGG pathway maps:

Graphical map objects

The KEGG pathway map is a moleculalr interaction/reaction network diagram represented in terms of the KEGG Orthology (KO) groups, so that experimental evidence in specific organisms can be generalized to other organisms through genomic information. Each map is manually drawn with in-house software called KegSketch, which generates the KGML+ file. This file is an SVG file containing graphics objects that are associated with KEGG objects (see KEGG object identifiers). Basic graphics objects in the reference KEGG pathway maps are:

boxes - ortholog (KO) groups identified by K numbers and, in metabolic maps, reactions identified by R numbers as well

circles - other molecules, usually chemical compounds identified by C numbers, but including glycans identified by G numbers

lines - reactions identified by R numbers in metabolic maps; ortholog (KO) groups identified by K numbers in global metabolism maps

and in organism specific pathway maps that are computationally generated:

boxes - genes or gene products identified by the combination of the KEGG organism code and gene identifiers

These map objects can be searched in the search box at the top of the KEGG PATHWAY page, in the search box in each pathway map, and by the KEGG Mapper tools.


Convention of map number prefix

Each pathway map is identified by the combination of 2-4 letter code and 5 digit number (see KEGG Identifiers). The prefix has the following meaning:

map - Reference pathway

ko - Reference pathway (KO)

ec - Reference pathway (EC)

rn - Reference pathway (Reaction)

org - Organism-specific pathway map

Only the first reference pathway map is manually drawn; all other maps are computationally generated. For metabolic pathways, each box (or line) in the reference map is linked to the K number (KO identifeir), the EC number, and the R number (reaction identifier). The KO, EC, and reaction maps are linked to only one of them. For all metabolic and non-metabolic maps, K numbers are converted to gene identifiers in each organism to generate organism-specific pathways.



As shown above, "map" pathways are not colored, "ko/ec/rn" pathways are colored blue, and organism-specific pathways are colored green, where coloring indicates that map objects exist and are linked to corresponding entries. For global metabolism maps, "map" pathways are fully colored, so that "ko/ec/rn" pathways and organism-specific pathways are generated by reducing the coloring indicating the absence of corresponding entries.

In this study, all KEGG pathway maps were deposited in summary/5_differential_expression/*VS*/3_KEGG_Pictures/.



5. Results of advanced analysis


5.1 SNP/Indel calling


5.1.1 Statistics of SNP/Indel


SampleSNVSNV(GeneRegion)INDELINDEL(GeneRegion)
Lo7_151301153155971825
Lo7_251834154356710950
Lo7_351936152606512959
Lo8_150456156206079925
Lo8_2510531581265091045
Lo8_354012162326382916

document location: summary/6_snp_and_Indel/1_SNV_INDEL_Statistics.xlsx


5.1.2 Statistics of SNP/Indel in position


Sample(SNV)intergenicintronicexonicUTR3UTR5upstreamdownstreamsplicingSample(INDEL)intergenicintronicexonicUTR3UTR5upstreamdownstreamsplicing
Lo7_1760613162171624860246226053174270Lo7_1631175495710148343514228
Lo7_2786212961173114881244028143270295Lo7_27022029110410168984574959
Lo7_3770513339171074932239728413328287Lo7_371619131105103284241547217
Lo8_1745811991174734881250226013262288Lo8_16511620105598491339045610
Lo8_2768911806177204909258528273252265Lo8_270617451181103692842647215
Lo8_3812313431181075026264529323469279Lo8_370417751064102590941247914

document location: summary/6_snp_and_Indel/2_SNV_INEDL_Position_Type.xlsx


5.1.3 Statistics of SNP/Indel typies


SNP_typeLo7_1Lo7_2Lo7_3Lo8_1Lo8_2Lo8_3
Transition321343309632750319803225834455
A-G158231622016163158371599017075
C-T163111687616587161431626817380
Transversion186761820318676179881824618986
A-C503348684954481649865161
A-T464546354689437943544625
C-G404440234212404640934234
G-T495446774821474748134966
Total508105129951426499685050453441

document location: summary/6_snp_and_Indel/3_SNP_Variation_Type.xlsx


5.1.4 Annotation for SNP/Indel


SNV(exonic)SynonymousNonSynonymousStopGainStopLossunknownINDEL(exonic)frameshift Insertionframeshift DeletionNonframeshift InsertionNonframeshift Deletionframeshift block substitutionnonframeshift block substitution
Lo7_1153156935769337828227Lo7_18258026221524900
Lo7_2154356998777135328726Lo7_29508637622024400
Lo7_3152606849773437027928Lo7_39596936421828200
Lo8_1156206985795339126427Lo8_19257231124127700
Lo8_2158127084803238728326Lo8_210459939424428100
Lo8_3162327327820339428325Lo8_39169230823525700

document location: summary/6_snp_and_Indel/4_SNV_INEDL_Annotation.xlsx



All SNPs/Indels were annotated by SnpEff in VCF format.


VCF format Description:

Term Description
CHROMchromosome id
POS IDchromosome position
REFreference allele
ALTalternative allele
QUALquality
FILTERfilter
INFOinformation
ADAllelic depths
DPApproximate read depth
GQGenotype Quality
GTgenotype
PLPhred-scaled likelihoods

document location:

summary/6_snp_and_Indel/6_INDEL_All_Samples.xlsx

summary/6_snp_and_Indel/*/*.vcf


5.2 Alternative splicing analysis


Alternative splicing, or differential splicing, is a regulated process during gene expression that results in a single gene coding for multiple proteins. In this process, particular exons of a gene may be included within or excluded from the final, processed messenger RNA (mRNA) produced from that gene. Consequently, the proteins translated from alternatively spliced mRNAs will contain differences in their amino acid sequence and, often, in their biological functions (see Figure). Notably, alternative splicing allows genome to direct the synthesis of many more proteins than would be expected from its 20,000 protein-coding genes.

Alternative splicing occurs as a normal phenomenon in eukaryotes, where it greatly increases the biodiversity of proteins that can be encoded by the genome; in humans, ~95% of multi-exonic genes are alternatively spliced.[2] There are numerous modes of alternative splicing observed, of which the most common is exon skipping. In this mode, a particular exon may be included in mRNAs under some conditions or in particular tissues, and omitted from the mRNA in others.


There are different kinds of alternative splicing including:

(A) Exon skipping (SKIP) and cassette exons (MSKIP)

(B) retention of single (IR) and multiple (MIR) introns

(C) alternative exon ends (AE)

(D) alternative transcription start site (TSS)

(E) alternative transcription termination site (TTS)

(F) Approximate SKIP (XSKIP)

(G) Approximate MSKIP (XMKIP)

(H) Approximate IR(XIR)

(I) Approximate MIR(XMIR)

(J) Approximate AE(XAE)



document location:

summary/7_AS_category_splicing/1_all_sample_AS_stat_histogram.png

summary/7_AS_category_splicing/1_AS_stat_all.xlsx



Alternative splicing in all samples


chromevent_typeevent_startevent_endevent_patternstrandgene_idtxpt_idAnnotationGOKEGGLo7_1(fpkm)Lo7_2(fpkm)Lo7_3(fpkm)Lo8_1(fpkm)Lo8_2(fpkm)Lo8_3(fpkm)
chr1AE100090541000914910009054-10009149-MSTRG.1019LOC_Os01g17396.1expressed protein3.121.592.061.671.361.32
chr1AE100090541000916410009054-10009164-MSTRG.1019LOC_Os01g17396.2expressed protein3.351.451.791.220.290.49
chr1AE100090541000916410009054-10009164-MSTRG.1019MSTRG.1019.11.581.583.730.400.960.08
chr1AE102716831027216810271683-10272168-MSTRG.1041MSTRG.1041.19.125.498.535.465.443.16
chr1AE102716831027218310271683-10272183-MSTRG.1041MSTRG.1041.32.031.481.531.590.691.76
chr1AE102716831027218310271683-10272183-MSTRG.1041MSTRG.1041.40.230.900.310.480.380.37
chr1AE103468981034692910346898-10346929-MSTRG.1050MSTRG.1050.80.501.4500.1500
chr1AE103468981034710710346898-10347107-MSTRG.1050MSTRG.1050.60.821.2200.4100
chr1AE103473981034748810347398-10347488-MSTRG.1050MSTRG.1050.30.2602.981.700.880
chr1AE103473981034748810347398-10347488-MSTRG.1050MSTRG.1050.50.0301.281.050.140
chr1AE103473981034821910347398-10348219-MSTRG.1050MSTRG.1050.12.9100.670.861.110
chr1AE103473981034821910347398-10348219-MSTRG.1050MSTRG.1050.40.3100.570.131.120
chr1AE103473981034821910347398-10348219-MSTRG.1050MSTRG.1050.70000.2200
chr1AE103484271034861410348427-10348614-MSTRG.1050MSTRG.1050.21.0300.3000.450
chr1AE103484271034861410348427-10348614-MSTRG.1050MSTRG.1050.60.8202.430.411.180
chr1AE103484271034861410348427-10348614-MSTRG.1050MSTRG.1050.80.50000.1500
chr1AE103484551034861410348455-10348614-MSTRG.1050LOC_Os01g18410.1expressed protein0.0400.41000
chr1AE103484551034861410348455-10348614-MSTRG.1050MSTRG.1050.30.2602.981.700.880
chr1AE103484551034861410348455-10348614-MSTRG.1050MSTRG.1050.50.0301.281.050.140
chr1AE103484551034896510348455-10348965-MSTRG.1050MSTRG.1050.12.9100.67000
chr1AE104946981049497210494698-10494972-MSTRG.1053MSTRG.1053.121.8400.30000.02
chr1AE104946981049504110494698-10495041-MSTRG.1053LOC_Os01g18630.1aspartic proteinase oryzasin-1 precursor, putative, expressedGO:0004190(aspartic-type endopeptidase activity),GO:0006508(proteolysis),GO:0030163(protein catabolic process)000005.88
chr1AE104946981049504110494698-10495041-MSTRG.1053LOC_Os01g18630.2aspartic proteinase oryzasin-1 precursor, putative, expressedGO:0004190(aspartic-type endopeptidase activity),GO:0006508(proteolysis),GO:0030163(protein catabolic process)0.1900.21004.39
chr1AE104946981049504110494698-10495041-MSTRG.1053MSTRG.1053.320.40016.82000
chr1AE104946981049504110494698-10495041-MSTRG.1053MSTRG.1053.420.20018.15000
chr1AE104946981049504110494698-10495041-MSTRG.1053MSTRG.1053.6000.00000
chr1AE104953931049568610495393-10495686-MSTRG.1053MSTRG.1053.132.870.2000.060.180
chr1AE104953931049579810495393-10495798-MSTRG.1053MSTRG.1053.901.520.12000
chr1AE104955331049568610495533-10495686-MSTRG.1053MSTRG.1053.12000.30000
chr1AE104955331049568610495533-10495686-MSTRG.1053MSTRG.1053.320.403.0916.8214.7818.150
chr1AE104955331049568610495533-10495686-MSTRG.1053MSTRG.1053.420.202.2818.1514.1616.700
chr1AE104955331049568610495533-10495686-MSTRG.1053MSTRG.1053.500003.570
chr1AE104955331049579810495533-10495798-MSTRG.1053MSTRG.1053.8010.170000
chr1AE104955331049580310495533-10495803-MSTRG.1053MSTRG.1053.6011.150.002.7100
chr1AE104955331049580310495533-10495803-MSTRG.1053MSTRG.1053.7014.15002.440
chr1AE105049810506241050498-1050624+MSTRG.134MSTRG.134.25.7610.139.176.296.6611.53
chr1AE105049810506241050498-1050624+MSTRG.134MSTRG.134.31.860.720.922.441.651.00
chr1AE105049810506241050498-1050624+MSTRG.134MSTRG.134.41.220.682.090.620.470.57
chr1AE105049810506241050498-1050624+MSTRG.134MSTRG.134.72.952.211.521.071.442.20
chr1AE105050810506241050508-1050624+MSTRG.134MSTRG.134.18.126.966.616.1110.1210.80
chr1AE105050810506241050508-1050624+MSTRG.134MSTRG.134.62.812.892.181.990.852.95
chr1AE105051110506241050511-1050624+MSTRG.134LOC_Os01g02890.1phosphatidylserine synthase, putative, expressedGO:0008654(phospholipid biosynthetic process),GO:0016021(integral component of membrane)00564(Glycerophospholipid metabolism)0.831.180.790.320.401.06
chr1AE105194710520331051947-1052033+MSTRG.134MSTRG.134.31.860.720.922.441.651.00
chr1AE105194710520331051947-1052033+MSTRG.134MSTRG.134.72.952.211.521.071.442.20
chr1AE105194710522001051947-1052200+MSTRG.134LOC_Os01g02890.1phosphatidylserine synthase, putative, expressedGO:0008654(phospholipid biosynthetic process),GO:0016021(integral component of membrane)00564(Glycerophospholipid metabolism)0.831.180.790.320.401.06
chr1AE105194710522001051947-1052200+MSTRG.134MSTRG.134.18.126.966.616.1110.1210.80
chr1AE105194710522001051947-1052200+MSTRG.134MSTRG.134.25.7610.139.176.296.6611.53
chr1AE105194710522001051947-1052200+MSTRG.134MSTRG.134.41.220.682.090.620.470.57
chr1AE105926310594331059263-1059433-MSTRG.133LOC_Os01g02900.1glycosyltransferase, putative, expressedGO:0005576(extracellular region),GO:0016757(transferase activity, transferring glycosyl groups)00514(Other types of O-glycan biosynthesis),00514(Other types of O-glycan biosynthesis)0.2600000.48
chr1AE105926310594621059263-1059462-MSTRG.133LOC_Os01g02900.2glycosyltransferase, putative, expressedGO:0005576(extracellular region),GO:0016757(transferase activity, transferring glycosyl groups)00514(Other types of O-glycan biosynthesis),00514(Other types of O-glycan biosynthesis)1.8100000.03
chr1AE106510101065165610651010-10651656+MSTRG.1058LOC_Os01g18850.3OsSPL1 - SBP-box gene family member, expressedGO:0003677(DNA binding),GO:0003700(transcription factor activity, sequence-specific DNA binding),GO:0005634(nucleus),GO:0006351(transcription, DNA-templated),GO:0006355(regulation of transcription, DNA-templated),GO:0046872(metal ion binding)04075(Plant hormone signal transduction),04075(Plant hormone signal transduction),04075(Plant hormone signal transduction),04075(Plant hormone signal transduction)3.937.085.6112.029.256.72
chr1AE106510101065165610651010-10651656+MSTRG.1058MSTRG.1058.11.48000.1704.81
chr1AE106510101065165610651010-10651656+MSTRG.1058MSTRG.1058.41.311.020.080.070.010.00
chr1AE106510951065165610651095-10651656+MSTRG.1058LOC_Os01g18850.1OsSPL1 - SBP-box gene family member, expressedGO:0003677(DNA binding),GO:0003700(transcription factor activity, sequence-specific DNA binding),GO:0005634(nucleus),GO:0006351(transcription, DNA-templated),GO:0006355(regulation of transcription, DNA-templated),GO:0046872(metal ion binding)04075(Plant hormone signal transduction),04075(Plant hormone signal transduction),04075(Plant hormone signal transduction),04075(Plant hormone signal transduction)0.340.520.680.930.710.32
chr1AE106543371065445410654337-10654454+MSTRG.1058LOC_Os01g18850.1OsSPL1 - SBP-box gene family member, expressedGO:0003677(DNA binding),GO:0003700(transcription factor activity, sequence-specific DNA binding),GO:0005634(nucleus),GO:0006351(transcription, DNA-templated),GO:0006355(regulation of transcription, DNA-templated),GO:0046872(metal ion binding)04075(Plant hormone signal transduction),04075(Plant hormone signal transduction),04075(Plant hormone signal transduction),04075(Plant hormone signal transduction)0.340.520.680.930.710.32
chr1AE106543371065445410654337-10654454+MSTRG.1058LOC_Os01g18850.2OsSPL1 - SBP-box gene family member, expressedGO:0003677(DNA binding),GO:0003700(transcription factor activity, sequence-specific DNA binding),GO:0005634(nucleus),GO:0006351(transcription, DNA-templated),GO:0006355(regulation of transcription, DNA-templated),GO:0046872(metal ion binding)04075(Plant hormone signal transduction),04075(Plant hormone signal transduction),04075(Plant hormone signal transduction),04075(Plant hormone signal transduction)0.240.062.780.950.360.03
chr1AE106543371065445410654337-10654454+MSTRG.1058LOC_Os01g18850.3OsSPL1 - SBP-box gene family member, expressedGO:0003677(DNA binding),GO:0003700(transcription factor activity, sequence-specific DNA binding),GO:0005634(nucleus),GO:0006351(transcription, DNA-templated),GO:0006355(regulation of transcription, DNA-templated),GO:0046872(metal ion binding)04075(Plant hormone signal transduction),04075(Plant hormone signal transduction),04075(Plant hormone signal transduction),04075(Plant hormone signal transduction)3.937.085.6112.029.256.72
chr1AE106543371065445410654337-10654454+MSTRG.1058LOC_Os01g18850.4OsSPL1 - SBP-box gene family member, expressedGO:0003677(DNA binding),GO:0003700(transcription factor activity, sequence-specific DNA binding),GO:0005634(nucleus),GO:0006351(transcription, DNA-templated),GO:0006355(regulation of transcription, DNA-templated),GO:0046872(metal ion binding)04075(Plant hormone signal transduction),04075(Plant hormone signal transduction),04075(Plant hormone signal transduction),04075(Plant hormone signal transduction)0.220.2400.851.630.10
chr1AE106543371065445410654337-10654454+MSTRG.1058MSTRG.1058.11.48000.1704.81
chr1AE106543371065445410654337-10654454+MSTRG.1058MSTRG.1058.51.270.112.810.310.560.03
chr1AE106544071065445410654407-10654454+MSTRG.1058MSTRG.1058.41.311.020.080.070.010.00
chr1AE106794451067966110679445-10679661+MSTRG.1060MSTRG.1060.50.590.350.690.240.740.09
chr1AE106795741067966110679574-10679661+MSTRG.1060MSTRG.1060.31.070.290.703.001.490.06
chr1AE108157891081589310815789-10815893-MSTRG.1063LOC_Os01g19140.1O-sialoglycoprotein endopeptidase, putative, expressedGO:0000408(EKC/KEOPS complex),GO:0002949(tRNA threonylcarbamoyladenosine modification)0.970.260.800.650.380.55
chr1AE108157891081590310815789-10815903-MSTRG.1063MSTRG.1063.22.012.622.561.821.291.93
chr1AE108260831082616610826083-10826166+MSTRG.1066LOC_Os01g19150.1CGMC_GSK.3 - CGMC includes CDA, MAPK, GSK3, and CLKC kinases, expressedGO:0004672(protein kinase activity),GO:0004674(protein serine/threonine kinase activity),GO:0005524(ATP binding),GO:0005634(nucleus),GO:0005829(cytosol),GO:0006468(protein phosphorylation),GO:0009651(response to salt stress),GO:0009933(meristem structural organization),GO:0016310(phosphorylation),GO:0042538(hyperosmotic salinity response),GO:1901002(positive regulation of response to salt stress)04931(Insulin resistance),04931(Insulin resistance),04931(Insulin resistance),04931(Insulin resistance),04931(Insulin resistance)26.3111.7320.8618.2917.7623.82
chr1AE108260831082616610826083-10826166+MSTRG.1066LOC_Os01g19150.3CGMC_GSK.3 - CGMC includes CDA, MAPK, GSK3, and CLKC kinases, expressedGO:0004672(protein kinase activity),GO:0004674(protein serine/threonine kinase activity),GO:0005524(ATP binding),GO:0005634(nucleus),GO:0005829(cytosol),GO:0006468(protein phosphorylation),GO:0009651(response to salt stress),GO:0009933(meristem structural organization),GO:0016310(phosphorylation),GO:0042538(hyperosmotic salinity response),GO:1901002(positive regulation of response to salt stress)04931(Insulin resistance),04931(Insulin resistance),04931(Insulin resistance),04931(Insulin resistance),04931(Insulin resistance)8.439.023.092.393.797.23
chr1AE108260831082616610826083-10826166+MSTRG.1066LOC_Os01g19150.5CGMC_GSK.3 - CGMC includes CDA, MAPK, GSK3, and CLKC kinases, expressedGO:0004672(protein kinase activity),GO:0004674(protein serine/threonine kinase activity),GO:0005524(ATP binding),GO:0005634(nucleus),GO:0005829(cytosol),GO:0006468(protein phosphorylation),GO:0009651(response to salt stress),GO:0009933(meristem structural organization),GO:0016310(phosphorylation),GO:0042538(hyperosmotic salinity response),GO:1901002(positive regulation of response to salt stress)04931(Insulin resistance),04931(Insulin resistance),04931(Insulin resistance),04931(Insulin resistance),04931(Insulin resistance)0.9501.230.860.424.77
chr1AE108261171082616610826117-10826166+MSTRG.1066LOC_Os01g19150.4CGMC_GSK.3 - CGMC includes CDA, MAPK, GSK3, and CLKC kinases, expressedGO:0004672(protein kinase activity),GO:0004674(protein serine/threonine kinase activity),GO:0005524(ATP binding),GO:0005634(nucleus),GO:0005829(cytosol),GO:0006468(protein phosphorylation),GO:0009651(response to salt stress),GO:0009933(meristem structural organization),GO:0016310(phosphorylation),GO:0042538(hyperosmotic salinity response),GO:1901002(positive regulation of response to salt stress)04931(Insulin resistance),04931(Insulin resistance),04931(Insulin resistance),04931(Insulin resistance),04931(Insulin resistance)1.277.600.530.300.330.10
chr1AE109310010933151093100-1093315-MSTRG.138LOC_Os01g02940.1glycosyltransferase protein, putative, expressedGO:0005576(extracellular region),GO:0016757(transferase activity, transferring glycosyl groups)00514(Other types of O-glycan biosynthesis),00514(Other types of O-glycan biosynthesis),00514(Other types of O-glycan biosynthesis),00514(Other types of O-glycan biosynthesis),00514(Other types of O-glycan biosynthesis)3.040.220.9800.290.06
chr1AE109310010933151093100-1093315-MSTRG.138MSTRG.138.60.170.617.560.230.080.51
chr1AE109310010933421093100-1093342-MSTRG.138LOC_Os01g02940.2glycosyltransferase protein, putative, expressedGO:0005576(extracellular region),GO:0016757(transferase activity, transferring glycosyl groups)00514(Other types of O-glycan biosynthesis),00514(Other types of O-glycan biosynthesis),00514(Other types of O-glycan biosynthesis),00514(Other types of O-glycan biosynthesis),00514(Other types of O-glycan biosynthesis)1.302.110.620.171.312.38
chr1AE109310010933551093100-1093355-MSTRG.138LOC_Os01g02940.3glycosyltransferase protein, putative, expressedGO:0005576(extracellular region),GO:0016757(transferase activity, transferring glycosyl groups)00514(Other types of O-glycan biosynthesis),00514(Other types of O-glycan biosynthesis),00514(Other types of O-glycan biosynthesis),00514(Other types of O-glycan biosynthesis),00514(Other types of O-glycan biosynthesis)1.721.320.831.710.691.27
chr1AE109612421096130010961242-10961300-MSTRG.1080MSTRG.1080.21.350.050.110.130.500.01
chr1AE109612421096131510961242-10961315-MSTRG.1080MSTRG.1080.10.270.451.481.870.821.91
chr1AE109811761098132010981176-10981320-MSTRG.1086LOC_Os01g19410.3expressed proteinGO:0003674(molecular_function),GO:0005634(nucleus),GO:0008150(biological_process),GO:0016021(integral component of membrane)17.750.127.307.453.2512.97
chr1AE109811761098132010981176-10981320-MSTRG.1086MSTRG.1086.1032.1617.8411.7616.340
chr1AE109811761098142310981176-10981423-MSTRG.1086MSTRG.1086.36.023.524.092.772.505.55
chr1AE109814001098142310981400-10981423-MSTRG.1086LOC_Os01g19410.2expressed proteinGO:0003674(molecular_function),GO:0005634(nucleus),GO:0008150(biological_process),GO:0016021(integral component of membrane)7.260.144.574.211.882.40
chr1AE109814001098142310981400-10981423-MSTRG.1086MSTRG.1086.217.0415.6413.548.5710.8514.74
chr1AE109814001098158110981400-10981581-MSTRG.1086LOC_Os01g19410.1expressed proteinGO:0003674(molecular_function),GO:0005634(nucleus),GO:0008150(biological_process),GO:0016021(integral component of membrane)7.972.246.174.684.497.28
chr1AE110031731100330711003173-11003307-MSTRG.1090LOC_Os01g19450.1ATP-citrate synthase subunit 1, putative, expressedGO:0003878(ATP citrate synthase activity),GO:0005524(ATP binding),GO:0005737(cytoplasm),GO:0005829(cytosol),GO:0005886(plasma membrane),GO:0006085(acetyl-CoA biosynthetic process),GO:0006629(lipid metabolic process),GO:0009346(citrate lyase complex),GO:0046872(metal ion binding)00020(Citrate cycle (TCA cycle)),00020(Citrate cycle (TCA cycle))42.5544.4930.6557.9048.3454.32
chr1AE110031731100330711003173-11003307-MSTRG.1090LOC_Os01g19450.2ATP-citrate synthase subunit 1, putative, expressedGO:0003878(ATP citrate synthase activity),GO:0005524(ATP binding),GO:0005737(cytoplasm),GO:0005829(cytosol),GO:0005886(plasma membrane),GO:0006085(acetyl-CoA biosynthetic process),GO:0006629(lipid metabolic process),GO:0009346(citrate lyase complex),GO:0046872(metal ion binding)00020(Citrate cycle (TCA cycle)),00020(Citrate cycle (TCA cycle))1.150.3702.5001.76
chr1AE110031731100341011003173-11003410-MSTRG.1090MSTRG.1090.30.000.0812.000.190.080.06
chr1AE110035661100366611003566-11003666-MSTRG.1090LOC_Os01g19450.2ATP-citrate synthase subunit 1, putative, expressedGO:0003878(ATP citrate synthase activity),GO:0005524(ATP binding),GO:0005737(cytoplasm),GO:0005829(cytosol),GO:0005886(plasma membrane),GO:0006085(acetyl-CoA biosynthetic process),GO:0006629(lipid metabolic process),GO:0009346(citrate lyase complex),GO:0046872(metal ion binding)00020(Citrate cycle (TCA cycle)),00020(Citrate cycle (TCA cycle))1.150.3702.5001.76
chr1AE110035661100368911003566-11003689-MSTRG.1090LOC_Os01g19450.1ATP-citrate synthase subunit 1, putative, expressedGO:0003878(ATP citrate synthase activity),GO:0005524(ATP binding),GO:0005737(cytoplasm),GO:0005829(cytosol),GO:0005886(plasma membrane),GO:0006085(acetyl-CoA biosynthetic process),GO:0006629(lipid metabolic process),GO:0009346(citrate lyase complex),GO:0046872(metal ion binding)00020(Citrate cycle (TCA cycle)),00020(Citrate cycle (TCA cycle))42.5544.49057.90054.32
chr1AE110035661100368911003566-11003689-MSTRG.1090MSTRG.1090.30.000.0800.1900.06
chr1AE110368251103699111036825-11036991-MSTRG.1092LOC_Os01g19470.1nodal modulator 1 precursor, putative, expressedGO:0005576(extracellular region),GO:0005774(vacuolar membrane),GO:0005783(endoplasmic reticulum),GO:0005794(Golgi apparatus),GO:0009505(plant-type cell wall),GO:0016021(integral component of membrane)0011.10000
chr1AE110368871103699111036887-11036991-MSTRG.1092MSTRG.1092.1005.28000
chr1AE111311781113123511131178-11131235+MSTRG.1099LOC_Os01g19630.1expressed protein00.080.35000
chr1AE111311781113126511131178-11131265+MSTRG.1099LOC_Os01g19630.2expressed protein03.083.85000
chr1AE111311781113126511131178-11131265+MSTRG.1099LOC_Os01g19630.3expressed protein02.511.35000
chr1AE111311781113126511131178-11131265+MSTRG.1099LOC_Os01g19630.4expressed protein01.290.64000
chr1AE111311781113126511131178-11131265+MSTRG.1099MSTRG.1099.102.331.72000
chr1AE111311781113126511131178-11131265+MSTRG.1099MSTRG.1099.202.613.15000
chr1AE111311781113126511131178-11131265+MSTRG.1099MSTRG.1099.300.850000
chr1AE111311781113126511131178-11131265+MSTRG.1099MSTRG.1099.400.360000
chr1AE111311781113126511131178-11131265+MSTRG.1099MSTRG.1099.501.331.57000
chr1AE112559011125630111255901-11256301-MSTRG.1110MSTRG.1110.11.3000.02001.31

document location:summary/7_AS_category_splicing/2_AS_All_sample.xlsx



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